Human Bikunin / alpha 1 Microglobulin ELISA Kit PicoKine®

About this product

Human Bikunin / alpha 1 Microglobulin ELISA Kit PicoKine® (96 Tests) . Quantitate Human AMBP in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1598

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 40 35 40 38 2.04 5.3% Sample 2 243 252 272 236 250 13.51 5.4% Sample 3 418 447 460 414 434 19.35 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 40 35 40 38 2.04 5.3% Sample 1 38 40 35 40 38 2.04 5.3% Sample 2 243 252 272 236 250 13.51 5.4% Sample 2 243 252 272 236 250 13.51 5.4% Sample 3 418 447 460 414 434 19.35 4.4% Sample 3 418 447 460 414 434 19.35 4.4%

Synonyms

Protein AMBP; Alpha-1-microglobulin; Protein HC; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge; Inter-alpha-trypsin inhibitor light chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-acid-rich protein; Trypstatin; AMBP; HCP; ITIL

Gene Name

AMBP

Gene ID

259

UniProt

P02760

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A206-N352

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha- trypsin inhibitor is present in plasma and urine.

Applications

ELISA

Field of Research

Cancer, Cardiovascular, Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin, Serum Proteins, Tumor Biomarkers

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 243 418 36 264 406 Standard deviation 2.35 16.03 25.08 2.48 18.48 26.2%7 CV (%) 6.2% 6.6% 6% 6.9% 7% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 243 418 36 264 406 Standard deviation 2.35 16.03 25.08 2.48 18.48 26.2%7 CV (%) 6.2% 6.6% 6% 6.9% 7% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 243 418 36 264 406 Mean (pg/ml) 38 243 418 36 264 406 Standard deviation 2.35 16.03 25.08 2.48 18.48 26.2%7 Standard deviation 2.35 16.03 25.08 2.48 18.48 26.2%7 CV (%) 6.2% 6.6% 6% 6.9% 7% 6.2% CV (%) 6.2% 6.6% 6% 6.9% 7% 6.2%

Sensitivity

<10 pg/mL

Function

Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Bikunin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Alpha-1-microglobulin/bikunin precursor

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.082 0.148 0.199 0.325 0.472 0.769 1.218 1.868 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.082 0.148 0.199 0.325 0.472 0.769 1.218 1.868 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.082 0.148 0.199 0.325 0.472 0.769 1.218 1.868 O.D. 0.082 0.148 0.199 0.325 0.472 0.769 1.218 1.868

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.