- Catalog number
- EK1637
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Lipoprotein-associated phospholipase A2 (Lp-PLA2) also known as platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45-kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7%
- Synonyms
- Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA (2) ; PAF 2-acylhydrolase; PLA2G7; PAFAH
- Gene Name
- PLA2G7
- Gene ID
- 7941
- UniProt
- Q13093
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: F22-N441
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Biotinylated polyclonal antibody from goat
- Tissue Specificity
- Plasma.
- Applications
- ELISA
- Field of Research
- Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Platelets, Secreted Molecules
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7%
- Sensitivity
- <20 pg/mL
- Function
- Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human PLA2G7
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Phospholipase A2 group VII
- Subcellular Location
- Secreted, extracellular space.
- Protein Name
- Platelet-activating factor acetylhydrolase
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
