- Catalog number
- EK1646
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Amine oxidase, copper containing 3, also known as vascular adhesion protein (VAP-1) and HPAO is an enzyme that in humans is encoded by the AOC3 gene on chromosome 17. This protein is a member of the semicarbazide-sensitive amine oxidase (SSAO) family and is associated with many vascular diseases. This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1035 1027 1045 1029 1034 7 0.6% Sample 2 5427 5489 4892 4736 5136 327.42 6.3% Sample 3 21122 20989 20645 21778 21133 410.79 1.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1035 1027 1045 1029 1034 7 0.6% Sample 1 1035 1027 1045 1029 1034 7 0.6% Sample 2 5427 5489 4892 4736 5136 327.42 6.3% Sample 2 5427 5489 4892 4736 5136 327.42 6.3% Sample 3 21122 20989 20645 21778 21133 410.79 1.9% Sample 3 21122 20989 20645 21778 21133 410.79 1.9%
- Synonyms
- Membrane primary amine oxidase
- Gene Name
- AOC3
- Gene ID
- 8639
- UniProt
- Q16853
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N2-N763
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Biotinylated polyclonal antibody from goat
- Tissue Specificity
- Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis. Isoform 2 seems to be the predominant transcript in fetal kidneys, fetal cartilage and fetal tonsils. The highest relative expression of isoform 2 occurs in skeletal muscle, heart, pancreas, kidney, and lung.
- Applications
- ELISA
- Field of Research
- Alzheimer's Disease, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Neurodegenerative Disease, Neurology Process, Neuroscience, Signal Transduction
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1035 5427 21122 1103 5962 23124 Standard deviation 72.45 260.49 1203.95 80.51 387.53 1364.31 CV (%) 7% 4.8% 5.7% 7.3% 6.5% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1035 5427 21122 1103 5962 23124 Standard deviation 72.45 260.49 1203.95 80.51 387.53 1364.31 CV (%) 7% 4.8% 5.7% 7.3% 6.5% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1035 5427 21122 1103 5962 23124 Mean (pg/ml) 1035 5427 21122 1103 5962 23124 Standard deviation 72.45 260.49 1203.95 80.51 387.53 1364.31 Standard deviation 72.45 260.49 1203.95 80.51 387.53 1364.31 CV (%) 7% 4.8% 5.7% 7.3% 6.5% 5.9% CV (%) 7% 4.8% 5.7% 7.3% 6.5% 5.9%
- Sensitivity
- <20 pg/mL
- Function
- Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human VAP-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Amine oxidase, copper containing 3
- Subcellular Location
- Cell membrane
- Protein Name
- Membrane primary amine oxidase
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.026 0.11 0.178 0.359 0.659 1.062 1.628 1.994 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.026 0.11 0.178 0.359 0.659 1.062 1.628 1.994 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.026 0.11 0.178 0.359 0.659 1.062 1.628 1.994 O.D. 0.026 0.11 0.178 0.359 0.659 1.062 1.628 1.994
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
