- Catalog number
- EK1652
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin (IL) -12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant rat IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 36 34 34 1.87 5.5% Sample 2 222 226 219 229 224 3.8 1.6% Sample 3 502 447 478 479 476 19.55 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 36 34 34 1.87 5.5% Sample 1 35 31 36 34 34 1.87 5.5% Sample 2 222 226 219 229 224 3.8 1.6% Sample 2 222 226 219 229 224 3.8 1.6% Sample 3 502 447 478 479 476 19.55 4.1% Sample 3 502 447 478 479 476 19.55 4.1%
- Synonyms
- Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Il12a
- Gene Name
- Il12a
- Gene ID
- 4405
- UniProt
- Q9R103
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: (M23 - S335) + (R23 - S215)
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 222 502 34 224 454 Standard deviation 1.92 15.76 27.61 2.07 19.93 29.05 CV (%) 5.5% 7.1% 5.5% 6.1% 8.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 222 502 34 224 454 Standard deviation 1.92 15.76 27.61 2.07 19.93 29.05 CV (%) 5.5% 7.1% 5.5% 6.1% 8.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 222 502 34 224 454 Mean (pg/ml) 35 222 502 34 224 454 Standard deviation 1.92 15.76 27.61 2.07 19.93 29.05 Standard deviation 1.92 15.76 27.61 2.07 19.93 29.05 CV (%) 5.5% 7.1% 5.5% 6.1% 8.9% 6.4% CV (%) 5.5% 7.1% 5.5% 6.1% 8.9% 6.4%
- Sensitivity
- <1.5 pg/mL
- Function
- Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat IL-12 (P70)
- Gene Name Synonym
- Interleukin 12A
- Subcellular Location
- Secreted .
- Protein Name
- Interleukin-12 subunit alpha
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.111 0.17 0.3 0.558 0.92 1.518 2.216 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.111 0.17 0.3 0.558 0.92 1.518 2.216 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.111 0.17 0.3 0.558 0.92 1.518 2.216 O.D. 0.044 0.111 0.17 0.3 0.558 0.92 1.518 2.216
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
