- Catalog number
- EK1665
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin (TSLP) . Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 155 180 182 156 168 12.77 7.6% Sample 2 962 986 889 968 951 37 3.8% Sample 3 6142 5679 5442 6137 5850 301.38 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 155 180 182 156 168 12.77 7.6% Sample 1 155 180 182 156 168 12.77 7.6% Sample 2 962 986 889 968 951 37 3.8% Sample 2 962 986 889 968 951 37 3.8% Sample 3 6142 5679 5442 6137 5850 301.38 5.1% Sample 3 6142 5679 5442 6137 5850 301.38 5.1%
- Synonyms
- Cytokine receptor-like factor 2; Cytokine receptor-like molecule 2; CRLM-2; Thymic stromal lymphopoietin protein receptor; TSLP receptor; Type I cytokine receptor delta 1; Crlf2; Crlm2; Tpte2; Tslpr
- Gene Name
- Crlf2
- Gene ID
- 57914
- UniProt
- Q8CII9
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A20-L233
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- High level of expression in liver, lung and testis. Also expressed in heart, brain, spleen, thymus and bone marrow. Highly expressed in progenitors and myeloid cells. Isoform 2 is expressed in primary hemotopoietic cells.
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 155 962 6142 150 902 6620 Standard deviation 9.76 74.07 405.37 11.85 71.25 450.16 CV (%) 7.9% 7.7% 6.6% 7.9% 7.9% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 155 962 6142 150 902 6620 Standard deviation 9.76 74.07 405.37 11.85 71.25 450.16 CV (%) 7.9% 7.7% 6.6% 7.9% 7.9% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 155 962 6142 150 902 6620 Mean (pg/ml) 155 962 6142 150 902 6620 Standard deviation 9.76 74.07 405.37 11.85 71.25 450.16 Standard deviation 9.76 74.07 405.37 11.85 71.25 450.16 CV (%) 7.9% 7.7% 6.6% 7.9% 7.9% 6.8% CV (%) 7.9% 7.7% 6.6% 7.9% 7.9% 6.8%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for thymic stromal lymphopoietin (TSLP) . Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse CRLF2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cytokine receptor-like factor 2
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.063 0.113 0.172 0.304 0.537 0.949 1.605 2.377 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.063 0.113 0.172 0.304 0.537 0.949 1.605 2.377 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.063 0.113 0.172 0.304 0.537 0.949 1.605 2.377 O.D. 0.063 0.113 0.172 0.304 0.537 0.949 1.605 2.377
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
