- Catalog number
- EK1660
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- NTRK3 (Neurotrophic Tyrosine Kinase Receptor Type 3), also known as TRKC, is a protein that in humans is encoded by the NTRK3 gene. By PCR analysis of a somatic cell hybrid panel and by fluorescence in situ hybridization with the cDNA clone, it mapped the NTRK3 gene to 15q24-q25. TRKC, a member of the TRK family of tyrosine protein kinase genes. They found that TRKC is preferentially expressed in the brain; in situ hybridization studies showed transcripts in the hippocampus, cerebral cortex, and the granular cell layer of the cerebellum. By functional studies in HeLa cells, 5 miRNAs regulate the truncated form of NTRK3.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 290 303 323 288 301 13.94 4.6% Sample 2 1865 1884 1959 1919 1906 35.84 1.8% Sample 3 4243 4177 4027 3835 4070 156.88 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 290 303 323 288 301 13.94 4.6% Sample 1 290 303 323 288 301 13.94 4.6% Sample 2 1865 1884 1959 1919 1906 35.84 1.8% Sample 2 1865 1884 1959 1919 1906 35.84 1.8% Sample 3 4243 4177 4027 3835 4070 156.88 3.8% Sample 3 4243 4177 4027 3835 4070 156.88 3.8%
- Synonyms
- NT-3 growth factor receptor; GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; Ntrk3; Trkc
- Gene Name
- Ntrk3
- Gene ID
- 29613
- UniProt
- Q03351
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: C32-T429
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Widely expressed, mainly in the nervous tissue.
- Applications
- ELISA
- Field of Research
- Cancer, Growth and Development, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and cell lysates.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 290 1865 4243 262 1846 4355 Standard deviation 11.6 134.28 182.44 13.88 138.45 226.46 CV (%) 4% 7.2% 4.3% 5.3% 7.5% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 290 1865 4243 262 1846 4355 Standard deviation 11.6 134.28 182.44 13.88 138.45 226.46 CV (%) 4% 7.2% 4.3% 5.3% 7.5% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 290 1865 4243 262 1846 4355 Mean (pg/ml) 290 1865 4243 262 1846 4355 Standard deviation 11.6 134.28 182.44 13.88 138.45 226.46 Standard deviation 11.6 134.28 182.44 13.88 138.45 226.46 CV (%) 4% 7.2% 4.3% 5.3% 7.5% 5.2% CV (%) 4% 7.2% 4.3% 5.3% 7.5% 5.2%
- Sensitivity
- <10 pg/mL
- Function
- Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (By similarity) . NTRK3 isoforms containing insertions within the kinase domain can autophosphorylate in response to NTF3/neurotrophin-3, but cannot mediate downstream phenotypic responses (PubMed:8494647, PubMed:8494648) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat TRKC
- Gene Name Synonym
- Neurotrophic receptor tyrosine kinase 3
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.075 0.139 0.252 0.478 0.891 1.517 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.075 0.139 0.252 0.478 0.891 1.517 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.075 0.139 0.252 0.478 0.891 1.517 2.024 O.D. 0.026 0.075 0.139 0.252 0.478 0.891 1.517 2.024
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
