- Catalog number
- EK1662
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-1 family member 10 is a protein that in humans is encoded by the IL1F10 gene. The protein encoded by thi s gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 215 206 177 200 14.13 7% Sample 2 449 456 455 464 456 5.33 1.1% Sample 3 1984 2038 1885 1780 1921 98.53 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 215 206 177 200 14.13 7% Sample 1 203 215 206 177 200 14.13 7% Sample 2 449 456 455 464 456 5.33 1.1% Sample 2 449 456 455 464 456 5.33 1.1% Sample 3 1984 2038 1885 1780 1921 98.53 5.1% Sample 3 1984 2038 1885 1780 1921 98.53 5.1%
- Synonyms
- Interleukin-1 family member 10; IL-1F10; Family of interleukin 1-theta; FIL1 theta; Interleukin-1 HY2; IL-1HY2; Interleukin-1 theta; IL-1 theta; Interleukin-38; IL-38; IL1F10; FIL1T; IL1HY2; IL38; FKSG75; UNQ6119/PRO20041
- Gene Name
- IL1F10
- Gene ID
- 84639
- UniProt
- Q8WWZ1
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: M1-W152
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed in fetal skin, spleen and tonsil. Expressed mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil.
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 449 1984 197 483 1980 Standard deviation 12.58 26.94 87.29 13.98 38.15 99 CV (%) 6.2% 6% 4.4% 7.1% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 449 1984 197 483 1980 Standard deviation 12.58 26.94 87.29 13.98 38.15 99 CV (%) 6.2% 6% 4.4% 7.1% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 203 449 1984 197 483 1980 Mean (pg/ml) 203 449 1984 197 483 1980 Standard deviation 12.58 26.94 87.29 13.98 38.15 99 Standard deviation 12.58 26.94 87.29 13.98 38.15 99 CV (%) 6.2% 6% 4.4% 7.1% 7.9% 5% CV (%) 6.2% 6% 4.4% 7.1% 7.9% 5%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS) . Ligand for IL-36R/IL1RL2.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human IL-38
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin 1 family member 10
- Subcellular Location
- Secreted.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.119 0.175 0.296 0.52 0.952 1.608 2.428 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.119 0.175 0.296 0.52 0.952 1.608 2.428 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.119 0.175 0.296 0.52 0.952 1.608 2.428 O.D. 0.061 0.119 0.175 0.296 0.52 0.952 1.608 2.428
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
