Human Serpin G1 ELISA Kit PicoKine®

About this product

Human Serpin G1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SERPING1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1667

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE) . Alternative splicing results in multiple transcript variants encoding the same isoform.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 209 218 225 15.73 6.9% Sample 2 1297 1249 1215 1306 1266 36.9 2.9% Sample 3 6095 6211 6061 5414 5945 311.71 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 209 218 225 15.73 6.9% Sample 1 222 251 209 218 225 15.73 6.9% Sample 2 1297 1249 1215 1306 1266 36.9 2.9% Sample 2 1297 1249 1215 1306 1266 36.9 2.9% Sample 3 6095 6211 6061 5414 5945 311.71 5.2% Sample 3 6095 6211 6061 5414 5945 311.71 5.2%

Synonyms

Plasma protease C1 inhibitor; C1 Inh; C1Inh; C1 esterase inhibitor; C1-inhibiting factor; Serpin G1; SERPING1; C1IN; C1NH

Gene Name

SERPING1

Gene ID

710

UniProt

P05155

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N23-A500

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Complement, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1297 60242 242 1418 6290 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 CV (%) 6.7% 4.9% 6% 7.6% 6.7% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1297 60242 242 1418 6290 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 CV (%) 6.7% 4.9% 6% 7.6% 6.7% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 1297 60242 242 1418 6290 Mean (pg/ml) 222 1297 60242 242 1418 6290 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 CV (%) 6.7% 4.9% 6% 7.6% 6.7% 6% CV (%) 6.7% 4.9% 6% 7.6% 6.7% 6%

Sensitivity

<10 pg/mL

Function

Activation of the C1 complex is under control of the C1- inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Serpin G1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Serpin family G member 1

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.116 0.18 0.309 0.521 0.861 1.448 2.201 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.116 0.18 0.309 0.521 0.861 1.448 2.201 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.116 0.18 0.309 0.521 0.861 1.448 2.201 O.D. 0.042 0.116 0.18 0.309 0.521 0.861 1.448 2.201

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.