- Catalog number
- EK1679
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 102 99 102 98 5.33 5.4% Sample 2 213 209 247 247 229 18.05 7.8% Sample 3 889 919 932 949 922 21.94 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 102 99 102 98 5.33 5.4% Sample 1 89 102 99 102 98 5.33 5.4% Sample 2 213 209 247 247 229 18.05 7.8% Sample 2 213 209 247 247 229 18.05 7.8% Sample 3 889 919 932 949 922 21.94 2.3% Sample 3 889 919 932 949 922 21.94 2.3%
- Synonyms
- Trefoil factor 3; Intestinal trefoil factor; rITF; Polypeptide P1.B; rP1.B; Tff3; Itf
- Gene Name
- Tff3
- Gene ID
- 25563
- UniProt
- Q03191
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A23-F81
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed in goblet cells of the intestines, and colon, in paraventricular hypothalamus and supraoptic nuclei. Weakly expressed in gastric epithelial cells (at protein level) . Expressed by goblet cells of small and large intestinal epithelia, kidney and stomach. Expressed in the paraventricular hypothalamus, arcuate nucleus and amygdala of the brain. Weakly expressed in gastric epithelial cells.
- Applications
- ELISA
- Field of Research
- Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 213 889 82 218 915 Standard deviation 5.76 12.99 50.67 6.8 17.55 53.98 CV (%) 6.7% 6.1% 5.7% 8.3% 7.5% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 213 889 82 218 915 Standard deviation 5.76 12.99 50.67 6.8 17.55 53.98 CV (%) 6.7% 6.1% 5.7% 8.3% 7.5% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 213 889 82 218 915 Mean (pg/ml) 89 213 889 82 218 915 Standard deviation 5.76 12.99 50.67 6.8 17.55 53.98 Standard deviation 5.76 12.99 50.67 6.8 17.55 53.98 CV (%) 6.7% 6.1% 5.7% 8.3% 7.5% 5.9% CV (%) 6.7% 6.1% 5.7% 8.3% 7.5% 5.9%
- Sensitivity
- <10 pg/mL
- Function
- Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) (By similarity) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat TFF3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Trefoil factor 3
- Subcellular Location
- Secreted, extracellular space, extracellular matrix.
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.103 0.162 0.254 0.383 0.658 1.082 1.886 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.103 0.162 0.254 0.383 0.658 1.082 1.886 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.103 0.162 0.254 0.383 0.658 1.082 1.886 O.D. 0.03 0.103 0.162 0.254 0.383 0.658 1.082 1.886
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
