- Catalog number
- EK1622
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 64 70 71 70 3.93 5.6% Sample 2 265 238 250 267 255 11.81 4.6% Sample 3 861 722 744 758 771 53.38 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 64 70 71 70 3.93 5.6% Sample 1 75 64 70 71 70 3.93 5.6% Sample 2 265 238 250 267 255 11.81 4.6% Sample 2 265 238 250 267 255 11.81 4.6% Sample 3 861 722 744 758 771 53.38 6.9% Sample 3 861 722 744 758 771 53.38 6.9%
- Synonyms
- Fatty acid-binding protein, intestinal; Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP; Fabp2; Fabpi
- Gene Name
- Fabp2
- Gene ID
- 14079
- UniProt
- P55050
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A2-E132
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed in the small intestine. Highest expression levels in the proximal ileum.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 265 861 69 288 910 Standard deviation 4.52 13.78 38.74 4.53 17.56 56.42 CV (%) 7.9% 5.2% 4.5% 8.6% 6.1% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 265 861 69 288 910 Standard deviation 4.52 13.78 38.74 4.53 17.56 56.42 CV (%) 7.9% 5.2% 4.5% 8.6% 6.1% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 75 265 861 69 288 910 Mean (pg/ml) 75 265 861 69 288 910 Standard deviation 4.52 13.78 38.74 4.53 17.56 56.42 Standard deviation 4.52 13.78 38.74 4.53 17.56 56.42 CV (%) 7.9% 5.2% 4.5% 8.6% 6.1% 6.2% CV (%) 7.9% 5.2% 4.5% 8.6% 6.1% 6.2%
- Sensitivity
- <10 pg/mL
- Function
- FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse FABP2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fatty acid binding protein 2, intestinal
- Subcellular Location
- Cytoplasm.
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.15 0.226 0.349 0.554 0.958 1.558 2.489 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.15 0.226 0.349 0.554 0.958 1.558 2.489 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.15 0.226 0.349 0.554 0.958 1.558 2.489 O.D. 0.051 0.15 0.226 0.349 0.554 0.958 1.558 2.489
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
