- Catalog number
- EK1623
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 131 127 121 131 127 4.09 3.2% Sample 2 866 923 850 898 884 28.26 3.1% Sample 3 2555 2241 2468 2352 2404 118.5 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 131 127 121 131 127 4.09 3.2% Sample 1 131 127 121 131 127 4.09 3.2% Sample 2 866 923 850 898 884 28.26 3.1% Sample 2 866 923 850 898 884 28.26 3.1% Sample 3 2555 2241 2468 2352 2404 118.5 4.9% Sample 3 2555 2241 2468 2352 2404 118.5 4.9%
- Synonyms
- Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Fabp3
- Gene Name
- Fabp3
- Gene ID
- 79131
- UniProt
- P07483
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A2-A133
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Heart, but also skeletal muscle, kidney, brain and mammary gland.
- Applications
- ELISA
- Field of Research
- Fatty Acids, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Mesenchymal Stem Cells, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 866 2555 124 868 2555 Standard deviation 6.02 54.55 158.41 7.19 60.76 181.4 CV (%) 4.6% 7%% 6.2% 5.8% 7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 866 2555 124 868 2555 Standard deviation 6.02 54.55 158.41 7.19 60.76 181.4 CV (%) 4.6% 7%% 6.2% 5.8% 7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 131 866 2555 124 868 2555 Mean (pg/ml) 131 866 2555 124 868 2555 Standard deviation 6.02 54.55 158.41 7.19 60.76 181.4 Standard deviation 6.02 54.55 158.41 7.19 60.76 181.4 CV (%) 4.6% 7%% 6.2% 5.8% 7% 7.1% CV (%) 4.6% 7%% 6.2% 5.8% 7% 7.1%
- Sensitivity
- <10 pg/mL
- Function
- FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat FABP3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fatty acid binding protein 3
- Subcellular Location
- Cytoplasm.
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.141 0.225 0.388 0.705 1.056 1.745 2.06 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.141 0.225 0.388 0.705 1.056 1.745 2.06 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.141 0.225 0.388 0.705 1.056 1.745 2.06 O.D. 0.038 0.141 0.225 0.388 0.705 1.056 1.745 2.06
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
