- Catalog number
- EK1636
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 220 258 259 253 247 16.03 6.4% Sample 2 1610 1483 1380 1552 1506 85.63 5.6% Sample 3 4490 4875 4487 4849 4675 186.97 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 220 258 259 253 247 16.03 6.4% Sample 1 220 258 259 253 247 16.03 6.4% Sample 2 1610 1483 1380 1552 1506 85.63 5.6% Sample 2 1610 1483 1380 1552 1506 85.63 5.6% Sample 3 4490 4875 4487 4849 4675 186.97 3.9% Sample 3 4490 4875 4487 4849 4675 186.97 3.9%
- Synonyms
- Uteroglobin; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1; Scgb1a1; Cc10; Ugb; Utg
- Gene Name
- Scgb1a1
- Gene ID
- 25575
- UniProt
- P17559
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S20-V96
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways) .
- Applications
- ELISA
- Field of Research
- Immunoglobulins, Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 220 1610 4490 241 1635 4849 Standard deviation 8.8 123.97 298.6%4 10.36 140.61 374.3%2 CV (%) 4% 7.7% 6.6% 4.3% 8.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 220 1610 4490 241 1635 4849 Standard deviation 8.8 123.97 298.6%4 10.36 140.61 374.3%2 CV (%) 4% 7.7% 6.6% 4.3% 8.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 220 1610 4490 241 1635 4849 Mean (pg/ml) 220 1610 4490 241 1635 4849 Standard deviation 8.8 123.97 298.6%4 10.36 140.61 374.3%2 Standard deviation 8.8 123.97 298.6%4 10.36 140.61 374.3%2 CV (%) 4% 7.7% 6.6% 4.3% 8.6% 7.8% CV (%) 4% 7.7% 6.6% 4.3% 8.6% 7.8%
- Sensitivity
- <10 pg/mL
- Function
- Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat SCGB1A1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Secretoglobin family 1A member 1
- Subcellular Location
- Secreted.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.113 0.154 0.245 0.453 0.766 1.328 2.053 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.113 0.154 0.245 0.453 0.766 1.328 2.053 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.113 0.154 0.245 0.453 0.766 1.328 2.053 O.D. 0.049 0.113 0.154 0.245 0.453 0.766 1.328 2.053
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
