Human B2M / beta 2 Microglobulin ELISA Kit PicoKine®

About this product

Human B2M / beta 2 Microglobulin ELISA Kit PicoKine® (96 Tests) . Quantitate Human B2M in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1691

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells) . In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 293 284 309 337 305 20.14 6.6% Sample 2 1070 1104 1006 1045 1056 35.77 3.3% Sample 3 5097 4645 4569 4545 4714 224.18 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 293 284 309 337 305 20.14 6.6% Sample 1 293 284 309 337 305 20.14 6.6% Sample 2 1070 1104 1006 1045 1056 35.77 3.3% Sample 2 1070 1104 1006 1045 1056 35.77 3.3% Sample 3 5097 4645 4569 4545 4714 224.18 4.7% Sample 3 5097 4645 4569 4545 4714 224.18 4.7%

Synonyms

Beta-2-microglobulin; Beta-2-microglobulin form pI 5.3; B2M; CDABP0092; HDCMA22P

Gene Name

B2M

Gene ID

567

UniProt

P61769

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Target protein isolated from human plasma

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cardiovascular, Serum Proteins, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1103 1070 5097 310 1103 4757 Standard deviation 21.97 62.06 219.17 26.97 69.48 285.42 CV (%) 7.5% 5.8% 4.3% 8.7% 6.3% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1103 1070 5097 310 1103 4757 Standard deviation 21.97 62.06 219.17 26.97 69.48 285.42 CV (%) 7.5% 5.8% 4.3% 8.7% 6.3% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1103 1070 5097 310 1103 4757 Mean (pg/ml) 1103 1070 5097 310 1103 4757 Standard deviation 21.97 62.06 219.17 26.97 69.48 285.42 Standard deviation 21.97 62.06 219.17 26.97 69.48 285.42 CV (%) 7.5% 5.8% 4.3% 8.7% 6.3% 6% CV (%) 7.5% 5.8% 4.3% 8.7% 6.3% 6%

Sensitivity

<10 pg/mL

Function

Component of the class I major histocompatibility complex (MHC) . Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553) .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human B2M

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Beta-2-microglobulin

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.077 0.144 0.27 0.52 0.919 1.552 2.101 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.077 0.144 0.27 0.52 0.919 1.552 2.101 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.077 0.144 0.27 0.52 0.919 1.552 2.101 O.D. 0.008 0.077 0.144 0.27 0.52 0.919 1.552 2.101

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.