- Catalog number
- EK0674
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cathepsin H is a protein that in humans is encoded by the CTSH gene. The protein encoded by this gene gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 372 315 318 335 335 22.68 6.7% Sample 2 987 987 1084 1153 1052 70.12 6.6% Sample 3 4095 4519 4622 4221 4364 214.12 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 372 315 318 335 335 22.68 6.7% Sample 1 372 315 318 335 335 22.68 6.7% Sample 2 987 987 1084 1153 1052 70.12 6.6% Sample 2 987 987 1084 1153 1052 70.12 6.6% Sample 3 4095 4519 4622 4221 4364 214.12 4.9% Sample 3 4095 4519 4622 4221 4364 214.12 4.9%
- Synonyms
- Pro-cathepsin H; Cathepsin H mini chain; Cathepsin H; Cathepsin H heavy chain; Cathepsin H light chain; CTSH; CPSB
- Gene Name
- CTSH
- Gene ID
- 1512
- UniProt
- P09668
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: A23-V335
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Alzheimer's Disease, Cell Adhesion Proteins, Membrane Proteins, Neurodegenerative Disease, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 372 987 40395 395 1022 3714 Standard deviation 16.74 68.1 1622.919 22.91 80.73 185.7 CV (%) 4.5% 6.9% 4.1% 5.8% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 372 987 40395 395 1022 3714 Standard deviation 16.74 68.1 1622.919 22.91 80.73 185.7 CV (%) 4.5% 6.9% 4.1% 5.8% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 372 987 40395 395 1022 3714 Mean (pg/ml) 372 987 40395 395 1022 3714 Standard deviation 16.74 68.1 1622.919 22.91 80.73 185.7 Standard deviation 16.74 68.1 1622.919 22.91 80.73 185.7 CV (%) 4.5% 6.9% 4.1% 5.8% 7.9% 5% CV (%) 4.5% 6.9% 4.1% 5.8% 7.9% 5%
- Sensitivity
- <10 pg/mL
- Function
- Important for the overall degradation of proteins in lysosomes.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human Cathepsin H
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cathepsin H
- Subcellular Location
- Lysosome.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.043 0.077 0.109 0.167 0.283 0.515 0.944 1.764 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.043 0.077 0.109 0.167 0.283 0.515 0.944 1.764 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.043 0.077 0.109 0.167 0.283 0.515 0.944 1.764 O.D. 0.043 0.077 0.109 0.167 0.283 0.515 0.944 1.764
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
