- Catalog number
- EK1692
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 94 96 98 95 1.47 1.5% Sample 2 317 320 299 298 308 10.06 3.2% Sample 3 1043 1012 1051 1042 1037 14.84 1.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 94 96 98 95 1.47 1.5% Sample 1 95 94 96 98 95 1.47 1.5% Sample 2 317 320 299 298 308 10.06 3.2% Sample 2 317 320 299 298 308 10.06 3.2% Sample 3 1043 1012 1051 1042 1037 14.84 1.4% Sample 3 1043 1012 1051 1042 1037 14.84 1.4%
- Synonyms
- Collagen alpha-1 (I) chain; Alpha-1 type I collagen; COL1A1
- Gene Name
- COL1A1
- Gene ID
- P02452
- UniProt
- P02452
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: Q23-K277 + G1094-L1464
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
- Applications
- ELISA
- Field of Research
- Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 360 1669 78 251 1684 Standard deviation 3.03 19.01 123.34 5.65 11.55 105.59 CV (%) 2.9% 5.4% 7.4% 7.2% 4.6% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 360 1669 78 251 1684 Standard deviation 3.03 19.01 123.34 5.65 11.55 105.59 CV (%) 2.9% 5.4% 7.4% 7.2% 4.6% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 360 1669 78 251 1684 Mean (pg/ml) 105 360 1669 78 251 1684 Standard deviation 3.03 19.01 123.34 5.65 11.55 105.59 Standard deviation 3.03 19.01 123.34 5.65 11.55 105.59 CV (%) 2.9% 5.4% 7.4% 7.2% 4.6% 6.3% CV (%) 2.9% 5.4% 7.4% 7.2% 4.6% 6.3%
- Sensitivity
- <10 pg/mL
- Function
- Type I collagen is a member of group I collagen (fibrillar forming collagen) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human COL1A1
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Collagen type I alpha 1 chain
- Subcellular Location
- Secreted, extracellular space, extracellular matrix
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.021 0.109 0.199 0.314 0.623 1.056 1.829 2.496 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.021 0.109 0.199 0.314 0.623 1.056 1.829 2.496 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.021 0.109 0.199 0.314 0.623 1.056 1.829 2.496 O.D. 0.021 0.109 0.199 0.314 0.623 1.056 1.829 2.496
- Activating Reagent
- Aliquot 0.1 ml per well of the 4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml human COL1A1 standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well) . Add 0.1 ml of each properly diluted sample of human cell culture supernatants, serum or plasma (heparin, EDTA, citrate) to each empty well. See “Sample Dilution Guideline” above for details. It is recommended that each human COL1A1 standard solution and each sample be measured in duplicate.
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
