- Catalog number
- EK1713
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Alpha-2-antiplasmin is a serine protease inhibitor (serpin) responsible for inactivating plasmin. Plasmin is an important enzyme that participates in fibrinolysis and degradation of various other proteins. This protein is encoded by the SERPINF2 gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2710 2230 2706 2277 2480 227.86 9.1% Sample .
2 13864 12175 13380 12105 12881 760.90 5.9%% Sample 3 42376 42947 47567 46317 44801 2194.70 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2710 2230 2706 2277 2480 227.86 9.1% Sample 1 2710 2230 2706 2277 2480 227.86 9.1% Sample .
2 13864 12175 13380 12105 12881 760.90 5.9%% Sample .
2 13864 12175 13380 12105 12881 760.90 5.9%% Sample 3 42376 42947 47567 46317 44801 2194.70 4.8% Sample 3 42376 42947 47567 46317 44801 2194.70 4.8%
- Synonyms
- Alpha-2-antiplasmin; Alpha-2-AP; Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2; Serpinf2; Pli
- Gene Name
- Serpinf2
- Gene ID
- 18816
- UniProt
- Q61247
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N40-K491
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed by the liver and secreted in plasma.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cardiovascular, Cell Biology, Neurogenesis, Neurology Process, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Sensory System, Visual System
- Standard
- 100 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 1.56 ng/mL - 100 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2710 13864 42376 2900 14200 45464 Standard deviation 113.82 804.1 2712.06 130.5 965.6 3273.4 CV (%) 4.2% 5.8% 6.4% 4.5% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2710 13864 42376 2900 14200 45464 Standard deviation 113.82 804.1 2712.06 130.5 965.6 3273.4 CV (%) 4.2% 5.8% 6.4% 4.5% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2710 13864 42376 2900 14200 45464 Mean (pg/ml) 2710 13864 42376 2900 14200 45464 Standard deviation 113.82 804.1 2712.06 130.5 965.6 3273.4 Standard deviation 113.82 804.1 2712.06 130.5 965.6 3273.4 CV (%) 4.2% 5.8% 6.4% 4.5% 6.8% 7.2% CV (%) 4.2% 5.8% 6.4% 4.5% 6.8% 7.2%
- Sensitivity
- <10 pg/mL
- Function
- Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse GPC3
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Serine (or cysteine) peptidase inhibitor, clade F, member 2
- Subcellular Location
- Secreted.
- Sample Data
- Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.019 0.077 0.154 0.342 0.6252 1.049 1.508 2.086 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.019 0.077 0.154 0.342 0.6252 1.049 1.508 2.086 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.019 0.077 0.154 0.342 0.6252 1.049 1.508 2.086 O.D. 0.019 0.077 0.154 0.342 0.6252 1.049 1.508 2.086
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
