- Catalog number
- EK1711
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
- Reproducibility
- Lots Lot 1 (ng/ml) Lot 2 (ng/ml) Lot 3 (ng/ml) Lot 4 (ng/ml) Mean (ng/ml) Standard Deviation CV (%) Sample 1 2876 2791 2438 2616 2680 168.37 6.2% Sample .
2 13987 13721 13461 14393 13890 344.60 2.4% Sample 3 37231 37794 38247 41965 38809 1857.18 4.7% Lots Lot 1 (ng/ml) Lot 2 (ng/ml) Lot 3 (ng/ml) Lot 4 (ng/ml) Mean (ng/ml) Standard Deviation CV (%) Lots Lot 1 (ng/ml) Lot 2 (ng/ml) Lot 3 (ng/ml) Lot 4 (ng/ml) Mean (ng/ml) Standard Deviation CV (%) Sample 1 2876 2791 2438 2616 2680 168.37 6.2% Sample 1 2876 2791 2438 2616 2680 168.37 6.2% Sample .
2 13987 13721 13461 14393 13890 344.60 2.4% Sample .
2 13987 13721 13461 14393 13890 344.60 2.4% Sample 3 37231 37794 38247 41965 38809 1857.18 4.7% Sample 3 37231 37794 38247 41965 38809 1857.18 4.7%
- Synonyms
- Serpina1c; Dom3; Dom6; Spi1-3; Spi1-6; Alpha-1-antitrypsin 1-3; Alpha-1 protease inhibitor 3; Alpha-1 protease inhibitor 6; Alpha-1-antitrypsin 1-6; Serine protease inhibitor 1-3; Serine protease inhibitor 1-6; Serine protease inhibitor A1c; Serpin A1c
- Gene Name
- Serpina1c
- Gene ID
- 20702
- UniProt
- Q00896
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E25-K413
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Surface Molecules, Transcription, Transcription Factors, Transcription Factors/Regulators
- Standard
- 100 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 1.56 ng/mL - 100 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5%
- Sensitivity
- <10 pg/mL
- Function
- Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse AAT
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Alpha-1-antitrypsin 1-3
- Sample Data
- Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.023 0.076 0.172 0.261 0.433 0.745 1.236 1.903 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.023 0.076 0.172 0.261 0.433 0.745 1.236 1.903 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.023 0.076 0.172 0.261 0.433 0.745 1.236 1.903 O.D. 0.023 0.076 0.172 0.261 0.433 0.745 1.236 1.903
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
