- Catalog number
- EK0694
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 311 305 362 322 23.20 7.2% Sample 2 1057 1075 989 1027 1037 32.58 3.1% Sample 3 4116 3778 3962 4374 4057 218.42 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 311 305 362 322 23.20 7.2% Sample 1 310 311 305 362 322 23.20 7.2% Sample 2 1057 1075 989 1027 1037 32.58 3.1% Sample 2 1057 1075 989 1027 1037 32.58 3.1% Sample 3 4116 3778 3962 4374 4057 218.42 5.3% Sample 3 4116 3778 3962 4374 4057 218.42 5.3%
- Synonyms
- Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14
- Gene Name
- CD14
- Gene ID
- 929
- UniProt
- P08571
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: T20-C352
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Detected on macrophages (at protein level) . Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Hematopoietic Progenitors, Immunology, Innate Immunity, Mesenchymal Stem Cells, Myeloid, Stem Cells, TLR Signaling
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1057 4116 312 1164 4732 Standard deviation 13.95 49.7 209.92 18.1 75.66 350.2 CV (%) 4.5% 4.7% 5.1% 5.8% 6.5% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1057 4116 312 1164 4732 Standard deviation 13.95 49.7 209.92 18.1 75.66 350.2 CV (%) 4.5% 4.7% 5.1% 5.8% 6.5% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 310 1057 4116 312 1164 4732 Mean (pg/ml) 310 1057 4116 312 1164 4732 Standard deviation 13.95 49.7 209.92 18.1 75.66 350.2 Standard deviation 13.95 49.7 209.92 18.1 75.66 350.2 CV (%) 4.5% 4.7% 5.1% 5.8% 6.5% 7.4% CV (%) 4.5% 4.7% 5.1% 5.8% 6.5% 7.4%
- Sensitivity
- <50 pg/mL
- Function
- Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MyD88, TIRAP and
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CD14
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD14 molecule
- Subcellular Location
- Secreted, Cell membrane, GPI-anchor, Golgi apparatus, Membrane raft
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.095 0.155 0.259 0.42 0.759 1.264 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.095 0.155 0.259 0.42 0.759 1.264 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.095 0.155 0.259 0.42 0.759 1.264 2.024 O.D. 0.033 0.095 0.155 0.259 0.42 0.759 1.264 2.024
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
