- Catalog number
- EK1760
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1098 1094 1079 1015 1071 33.38 3.1% Sample 2 3228 3312 3233 3315 3272 41.55 1.2% Sample 3 15773 17166 16982 16097 16504 584.53 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1098 1094 1079 1015 1071 33.38 3.1% Sample 1 1098 1094 1079 1015 1071 33.38 3.1% Sample 2 3228 3312 3233 3315 3272 41.55 1.2% Sample 2 3228 3312 3233 3315 3272 41.55 1.2% Sample 3 15773 17166 16982 16097 16504 584.53 3.5% Sample 3 15773 17166 16982 16097 16504 584.53 3.5%
- Synonyms
- Basement membrane-specific heparan sulfate proteoglycan core protein; HSPG; Perlecan; PLC; HSPG2
- Gene Name
- HSPG2
- Gene ID
- 3339
- UniProt
- P98160
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E3687-S4391
- Capture Antibody
- A polyclonal antibody from goat
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Found in the basement membranes.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction
- Standard
- 40 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 0.624 ng/mL - 40 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1098 3228 15773 1118 4325 18932 Standard deviation 58.19 132.3 1056.8 60.4 311.4 1438.83 CV (%) 5.3% 4.1% 6.7% 5.4% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1098 3228 15773 1118 4325 18932 Standard deviation 58.19 132.3 1056.8 60.4 311.4 1438.83 CV (%) 5.3% 4.1% 6.7% 5.4% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1098 3228 15773 1118 4325 18932 Mean (pg/ml) 1098 3228 15773 1118 4325 18932 Standard deviation 58.19 132.3 1056.8 60.4 311.4 1438.83 Standard deviation 58.19 132.3 1056.8 60.4 311.4 1438.83 CV (%) 5.3% 4.1% 6.7% 5.4% 7.2% 7.6% CV (%) 5.3% 4.1% 6.7% 5.4% 7.2% 7.6%
- Sensitivity
- <0.05 ng/mL
- Function
- Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachm
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human Endorepellin/HSPG2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Heparan sulfate proteoglycan 2
- Subcellular Location
- Basement membrane
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.063 0.091 0.128 0.201 0.354 0.654 1.194 1.885 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.063 0.091 0.128 0.201 0.354 0.654 1.194 1.885 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.063 0.091 0.128 0.201 0.354 0.654 1.194 1.885 O.D. 0.063 0.091 0.128 0.201 0.354 0.654 1.194 1.885
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
