- Catalog number
- EK1759
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. This gene is mapped to 10q26.2. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 17 17 15 16 1.00 6.2% Sample 2 54 64 64 54 59 5.00 8.4% Sample 3 222 213 228 245 227 11.68 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 17 17 15 16 1.00 6.2% Sample 1 15 17 17 15 16 1.00 6.2% Sample 2 54 64 64 54 59 5.00 8.4% Sample 2 54 64 64 54 59 5.00 8.4% Sample 3 222 213 228 245 227 11.68 5.1% Sample 3 222 213 228 245 227 11.68 5.1%
- Synonyms
- Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; MKI67
- Gene Name
- MKI67
- Gene ID
- 4288
- UniProt
- P46013
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: N3120-I3256
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) . Present at highest level in G2 phase and during mitosis (at protein level) . In interphase
- Applications
- ELISA
- Field of Research
- Tumor Biomarkers
- Standard
- 1 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 7.8 pg/mL - 500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 54 222 16 60 234 Standard deviation 0.9 3.13 10.4 0.94 4.2 18.25 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 54 222 16 60 234 Standard deviation 0.9 3.13 10.4 0.94 4.2 18.25 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 54 222 16 60 234 Mean (pg/ml) 15 54 222 16 60 234 Standard deviation 0.9 3.13 10.4 0.94 4.2 18.25 Standard deviation 0.9 3.13 10.4 0.94 4.2 18.25 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8%
- Sensitivity
- <1 pg/mL
- Function
- Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome su
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human Ki67
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Marker of proliferation Ki-67
- Subcellular Location
- Nucleus , nucleolus, Chromosome
- Sample Data
- Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.2 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.2 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.2 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.2
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
