- Catalog number
- EK1753
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 16p13.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 47 51 54 49 2.94 6.0% Sample 2 209 195 217 190 202 10.77 5.3% Sample 3 855 760 804 881 825 46.64 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 47 51 54 49 2.94 6.0% Sample 1 47 47 51 54 49 2.94 6.0% Sample 2 209 195 217 190 202 10.77 5.3% Sample 2 209 195 217 190 202 10.77 5.3% Sample 3 855 760 804 881 825 46.64 5.6% Sample 3 855 760 804 881 825 46.64 5.6%
- Synonyms
- Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; Tweak-receptor; TweakR; CD266; TNFRSF12A; FN14
- Gene Name
- TNFRSF12A
- Gene ID
- 51330
- UniProt
- Q9NP84
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: E28-P80
- Capture Antibody
- The capture antibody is polyclonal antibody from goat
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 209 855 58 247 903 Standard deviation 2.21 11.5 50.45 3.71 16.55 67.8 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 209 855 58 247 903 Standard deviation 2.21 11.5 50.45 3.71 16.55 67.8 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 47 209 855 58 247 903 Mean (pg/ml) 47 209 855 58 247 903 Standard deviation 2.21 11.5 50.45 3.71 16.55 67.8 Standard deviation 2.21 11.5 50.45 3.71 16.55 67.8 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human TNFRSF12A/TWEAKR
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- TNF receptor superfamily member 12A
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.21 0.299 0.431 0.641 0.929 1.346 1.963 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.21 0.299 0.431 0.641 0.929 1.346 1.963 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.21 0.299 0.431 0.641 0.929 1.346 1.963 O.D. 0.029 0.21 0.299 0.431 0.641 0.929 1.346 1.963
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
