- Catalog number
- EK1771
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. This gene is mapped to 12q12. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 346 337 354 335 19.01 5.6% Sample 2 1040 1033 1054 1079 1051 17.58 1.6% Sample 3 4977 4553 5102 5081 4928 221.76 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 346 337 354 335 19.01 5.6% Sample 1 304 346 337 354 335 19.01 5.6% Sample 2 1040 1033 1054 1079 1051 17.58 1.6% Sample 2 1040 1033 1054 1079 1051 17.58 1.6% Sample 3 4977 4553 5102 5081 4928 221.76 4.5% Sample 3 4977 4553 5102 5081 4928 221.76 4.5%
- Synonyms
- Contactin-1; Glycoprotein gp135; Neural cell surface protein F3; CNTN1
- Gene Name
- CNTN1
- Gene ID
- 1272
- UniProt
- Q12860
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E21-S993
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1040 4977 310 1115 4983 Standard deviation 19.15 59.28 238.9 20.46 81.4 383.7 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1040 4977 310 1115 4983 Standard deviation 19.15 59.28 238.9 20.46 81.4 383.7 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 304 1040 4977 310 1115 4983 Mean (pg/ml) 304 1040 4977 310 1115 4983 Standard deviation 19.15 59.28 238.9 20.46 81.4 383.7 Standard deviation 19.15 59.28 238.9 20.46 81.4 383.7 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7%
- Sensitivity
- <50 pg/mL
- Function
- Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association w
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human Contactin-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Contactin 1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.066 0.105 0.186 0.336 0.598 1.116 1.898 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.066 0.105 0.186 0.336 0.598 1.116 1.898 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.066 0.105 0.186 0.336 0.598 1.116 1.898 O.D. 0.022 0.066 0.105 0.186 0.336 0.598 1.116 1.898
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
