- Catalog number
- EK1755
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. This gene is mapped to 3p12.3. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 115 97 115 109 7.46 6.8% Sample 2 381 396 415 397 397 12.04 3.0% Sample 3 1617 1731 1808 1764 1730 70.72 4.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 115 97 115 109 7.46 6.8% Sample 1 112 115 97 115 109 7.46 6.8% Sample 2 381 396 415 397 397 12.04 3.0% Sample 2 381 396 415 397 397 12.04 3.0% Sample 3 1617 1731 1808 1764 1730 70.72 4.0% Sample 3 1617 1731 1808 1764 1730 70.72 4.0%
- Synonyms
- Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1
- Gene Name
- ROBO1
- Gene ID
- 6091
- UniProt
- Q9Y6N7
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q26-P897
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Widely expressed, with exception of kidney.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Axonal Guidance Proteins, Cancer, Growth and Development, Invasion/Microenvironment, Neurogenesis, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 381 1617 121 423 1768 Standard deviation 5.71 20.4 69.53 5.93 28.3 139.7 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 381 1617 121 423 1768 Standard deviation 5.71 20.4 69.53 5.93 28.3 139.7 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 381 1617 121 423 1768 Mean (pg/ml) 112 381 1617 121 423 1768 Standard deviation 5.71 20.4 69.53 5.93 28.3 139.7 Standard deviation 5.71 20.4 69.53 5.93 28.3 139.7 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human ROBO1
- Anticoagulant
- Heparin
- Gene Name Synonym
- Roundabout guidance receptor 1
- Subcellular Location
- Cell membrane, Single-pass type I membrane protein, axon
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.08 0.153 0.274 0.49 0.896 1.512 2.129 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.08 0.153 0.274 0.49 0.896 1.512 2.129 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.08 0.153 0.274 0.49 0.896 1.512 2.129 O.D. 0.007 0.08 0.153 0.274 0.49 0.896 1.512 2.129
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
