- Catalog number
- EK1811
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Sialomucin core protein 24 also known as endolyn or CD164, is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 149 135 144 154 145 7.01 4.8% Sample 2 717 672 786 731 726 40.68 5.6% Sample 3 2964 3060 3039 3034 3049 62.59 2.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 149 135 144 154 145 7.01 4.8% Sample 1 149 135 144 154 145 7.01 4.8% Sample 2 717 672 786 731 726 40.68 5.6% Sample 2 717 672 786 731 726 40.68 5.6% Sample 3 2964 3060 3039 3034 3049 62.59 2.0% Sample 3 2964 3060 3039 3034 3049 62.59 2.0%
- Synonyms
- Sialomucin core protein 24; MUC-24; Endolyn; Multi-glycosylated core protein 24; MGC-24; MGC-24v; CD164
- Gene Name
- CD164
- Gene ID
- 8763
- UniProt
- Q04900
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D24-D162
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Isoform 1 and isoform 3 are expressed in hematopoietic and non-hematopoietic tissues. Isoform 1 is expressed by prostate cancer tumors and prostate cancer cell lines. The expression is greater in bone metastases than in primary tumors. Expression in osseo
- Applications
- ELISA
- Field of Research
- Extracellular Matrix, Signal Transduction, Tags & Cell Markers, Tumor Associated, Tumor Immunology, Tumor-Associated Antigens
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 93.8 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 149 717 2964 162 741 2982 Standard deviation 8.2 29.4 168.95 10.04 48.17 217.7 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 149 717 2964 162 741 2982 Standard deviation 8.2 29.4 168.95 10.04 48.17 217.7 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 149 717 2964 162 741 2982 Mean (pg/ml) 149 717 2964 162 741 2982 Standard deviation 8.2 29.4 168.95 10.04 48.17 217.7 Standard deviation 8.2 29.4 168.95 10.04 48.17 217.7 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3%
- Sensitivity
- <50 pg/mL
- Function
- Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38 (lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediat
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CD164
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD164 molecule
- Subcellular Location
- Cell membrane, Single-pass type I membrane protein, Endosome membrane, Lysosome membrane, Secreted
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.028 0.167 0.301 0.464 0.745 1.197 1.752 2.149 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.028 0.167 0.301 0.464 0.745 1.197 1.752 2.149 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.028 0.167 0.301 0.464 0.745 1.197 1.752 2.149 O.D. 0.028 0.167 0.301 0.464 0.745 1.197 1.752 2.149
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
