- Catalog number
- EK1815
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- This gene is mapped to 8; 8 C5. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. Enzyme that hydrolizes long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the elimination of toxic substances. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 257 282 286 273 274 11.14 4.0% Sample 2 1095 1002 969 1048 1028 47.55 4.6% Sample 3 4121 3854 4005 4025 4001 95.65 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 257 282 286 273 274 11.14 4.0% Sample 1 257 282 286 273 274 11.14 4.0% Sample 2 1095 1002 969 1048 1028 47.55 4.6% Sample 2 1095 1002 969 1048 1028 47.55 4.6% Sample 3 4121 3854 4005 4025 4001 95.65 2.3% Sample 3 4121 3854 4005 4025 4001 95.65 2.3%
- Synonyms
- Carboxylesterase 1D; Carboxylesterase 3; Fatty acid ethyl ester synthase; FAEE synthase; Triacylglycerol hydrolase; TGH; Ces1d; Ces1; Ces3
- Gene Name
- Ces1d
- Gene ID
- 104158
- UniProt
- Q8VCT4
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Y19-L565
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Highest expression occurs in liver with lower levels in adipose tissue, kidney, heart, intestine, lung, testis and thymus.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 257 1095 4121 303 1263 4314 Standard deviation 15.93 71.2 177.2 15.76 77.04 310.6 CV (%) 6.2% 6.5% 4.3% 5.2% 6.1% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 257 1095 4121 303 1263 4314 Standard deviation 15.93 71.2 177.2 15.76 77.04 310.6 CV (%) 6.2% 6.5% 4.3% 5.2% 6.1% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 257 1095 4121 303 1263 4314 Mean (pg/ml) 257 1095 4121 303 1263 4314 Standard deviation 15.93 71.2 177.2 15.76 77.04 310.6 Standard deviation 15.93 71.2 177.2 15.76 77.04 310.6 CV (%) 6.2% 6.5% 4.3% 5.2% 6.1% 7.2% CV (%) 6.2% 6.5% 4.3% 5.2% 6.1% 7.2%
- Sensitivity
- <50 pg/mL
- Function
- Major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decre
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse CES3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Carboxylesterase 1D
- Subcellular Location
- Endoplasmic reticulum lumen, cytosol, Lipid droplet
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.1 0.183 0.357 0.676 1.161 1.703 1.928 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.1 0.183 0.357 0.676 1.161 1.703 1.928 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.1 0.183 0.357 0.676 1.161 1.703 1.928 O.D. 0.025 0.1 0.183 0.357 0.676 1.161 1.703 1.928
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
