- Catalog number
- EK1834
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Regenerating islet-derived protein 3 alpha (or Regenerating islet-derived protein III-alpha) formerly known as HIP/PAP (Hepatocarcinoma-Intestine-Pancreas/Pancreatitis-Associated Protein) and peptide 23 is a protein that in humans is encoded by the REG3A gene. This gene is mapped to 2p12. It encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1176 1259 1226 1140 1200 45.64 3.8% Sample 2 6316 7061 7332 7319 7007 413.32 5.8% Sample 3 19450 23051 22840 23122 22115 1542.56 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1176 1259 1226 1140 1200 45.64 3.8% Sample 1 1176 1259 1226 1140 1200 45.64 3.8% Sample 2 6316 7061 7332 7319 7007 413.32 5.8% Sample 2 6316 7061 7332 7319 7007 413.32 5.8% Sample 3 19450 23051 22840 23122 22115 1542.56 6.9% Sample 3 19450 23051 22840 23122 22115 1542.56 6.9%
- Synonyms
- Regenerating islet-derived protein 3-alpha; REG-3-alpha; Hepatointestinal pancreatic protein; HIP; PAP; Human proislet peptide; Pancreatitis-associated protein 1; Regenerating islet-derived protein III-alpha; Reg III-alpha; REG3A; PAP1
- Gene Name
- REG3A
- Gene ID
- 5068
- UniProt
- Q06141
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E27-D175
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Highly expressed in epidermal keratinocytes of psoriasis patients (at protein level) . Constitutively expressed in intestine. Low expression is found in healthy pancreas. Overexpressed during the acute phase of pancreatitis and in some patients with chroni
- Applications
- ELISA
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1176 6316 19450 1433 6401 19872 Standard deviation 58.80 385.28 836.35 65.92 409.66 1430.78 CV (%) 5.0% 6.1% 4.3% 4.6% 6.4% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1176 6316 19450 1433 6401 19872 Standard deviation 58.80 385.28 836.35 65.92 409.66 1430.78 CV (%) 5.0% 6.1% 4.3% 4.6% 6.4% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1176 6316 19450 1433 6401 19872 Mean (pg/ml) 1176 6316 19450 1433 6401 19872 Standard deviation 58.80 385.28 836.35 65.92 409.66 1430.78 Standard deviation 58.80 385.28 836.35 65.92 409.66 1430.78 CV (%) 5.0% 6.1% 4.3% 4.6% 6.4% 7.2% CV (%) 5.0% 6.1% 4.3% 4.6% 6.4% 7.2%
- Sensitivity
- <0.1 ng/mL
- Function
- Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injur
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human REG3A
- Anticoagulant
- Heparin
- Gene Name Synonym
- Regenerating family member 3 alpha
- Subcellular Location
- Secreted
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.078 0.148 0.189 0.261 0.456 0.762 1.223 1.982 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.078 0.148 0.189 0.261 0.456 0.762 1.223 1.982 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.078 0.148 0.189 0.261 0.456 0.762 1.223 1.982 O.D. 0.078 0.148 0.189 0.261 0.456 0.762 1.223 1.982
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
