- Catalog number
- EK1837
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Ly6/PLAUR domain-containing protein 3 is a protein that in humans is encoded by the LYPD3 gene. This gene is mapped to 19q13.31.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 604 542 577 23.17 4.0% Sample 2 2019 2207 2161 2108 2123 69.89 3.2% Sample 3 10613 9014 10567 8909 9775 815.25 8.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 604 542 577 23.17 4.0% Sample 1 590 572 604 542 577 23.17 4.0% Sample 2 2019 2207 2161 2108 2123 69.89 3.2% Sample 2 2019 2207 2161 2108 2123 69.89 3.2% Sample 3 10613 9014 10567 8909 9775 815.25 8.3% Sample 3 10613 9014 10567 8909 9775 815.25 8.3%
- Synonyms
- Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A homolog; Matrigel-induced gene C4 protein; MIG-C4; LYPD3; C4.4A
- Gene Name
- LYPD3
- Gene ID
- 27076
- UniProt
- O95274
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L31-C326
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TC
- Applications
- ELISA
- Field of Research
- Cancer, Tumor Biomarkers
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 2019 10613 621 2312 11100 Standard deviation 25.96 102.97 583.72 40.99 171.09 843.60 CV (%) 4.4% 5.1% 5.5% 6.6% 7.4% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 2019 10613 621 2312 11100 Standard deviation 25.96 102.97 583.72 40.99 171.09 843.60 CV (%) 4.4% 5.1% 5.5% 6.6% 7.4% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 590 2019 10613 621 2312 11100 Mean (pg/ml) 590 2019 10613 621 2312 11100 Standard deviation 25.96 102.97 583.72 40.99 171.09 843.60 Standard deviation 25.96 102.97 583.72 40.99 171.09 843.60 CV (%) 4.4% 5.1% 5.5% 6.6% 7.4% 7.6% CV (%) 4.4% 5.1% 5.5% 6.6% 7.4% 7.6%
- Sensitivity
- <0.1 ng/mL
- Function
- Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human LYPD3
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- LY6/PLAUR domain containing 3
- Subcellular Location
- Cell membrane, GPI-anchor
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.075 0.117 0.189 0.355 0.549 0.995 1.926 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.075 0.117 0.189 0.355 0.549 0.995 1.926 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.075 0.117 0.189 0.355 0.549 0.995 1.926 O.D. 0.031 0.075 0.117 0.189 0.355 0.549 0.995 1.926
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
