Human Plexin B2/PLXNB2 ELISA Kit PicoKine®

About this product

Human Plexin B2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human PLXNB2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1826

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.This gene is mapped to 22q13.33.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1214 1018 1165 1017 1103 87.72 7.9% Sample 2 4275 4570 4511 4741 4524 166.86 3.6% Sample 3 19603 18628 19571 18283 19021 578.86 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1214 1018 1165 1017 1103 87.72 7.9% Sample 1 1214 1018 1165 1017 1103 87.72 7.9% Sample 2 4275 4570 4511 4741 4524 166.86 3.6% Sample 2 4275 4570 4511 4741 4524 166.86 3.6% Sample 3 19603 18628 19571 18283 19021 578.86 3.0% Sample 3 19603 18628 19571 18283 19021 578.86 3.0%

Synonyms

Plexin-B2; MM1; PLXNB2; KIAA0315

Gene Name

PLXNB2

Gene ID

23654

UniProt

O15031

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-L1838

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Type Marker, Growth and Development, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

0.624 ng/mL - 40 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1214 4275 19603 1233 4407 19741 Standard deviation 63.13 239.4 960.55 82.6 339.3 1539.8 CV (%) 5.2% 5.6% 4.9% 6.7% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1214 4275 19603 1233 4407 19741 Standard deviation 63.13 239.4 960.55 82.6 339.3 1539.8 CV (%) 5.2% 5.6% 4.9% 6.7% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1214 4275 19603 1233 4407 19741 Mean (pg/ml) 1214 4275 19603 1233 4407 19741 Standard deviation 63.13 239.4 960.55 82.6 339.3 1539.8 Standard deviation 63.13 239.4 960.55 82.6 339.3 1539.8 CV (%) 5.2% 5.6% 4.9% 6.7% 7.7% 7.8% CV (%) 5.2% 5.6% 4.9% 6.7% 7.7% 7.8%

Sensitivity

<0.1 ng/mL

Function

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Plexin B2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Plexin B2

Subcellular Location

Cell membrane, Single-pass type I membrane protein

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.054 0.124 0.174 0.33 0.54 0.935 1.605 2.239 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.054 0.124 0.174 0.33 0.54 0.935 1.605 2.239 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.054 0.124 0.174 0.33 0.54 0.935 1.605 2.239 O.D. 0.054 0.124 0.174 0.33 0.54 0.935 1.605 2.239

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.