- Catalog number
- EK1827
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Plexin-B3 is a protein that in humans is encoded by the PLXNB3 gene. Ths gene is mapped to Xq28. The protein encoded by this gene is a member of the plexin family. It functions as a receptor for semaphorin 5A, and plays a role in axon guidance, invasive growth and cell migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 156 141 147 153 149 5.76 3.8% Sample 2 984 848 932 931 923 48.70 5.2% Sample 3 3201 3180 3318 2761 3115 211.03 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 156 141 147 153 149 5.76 3.8% Sample 1 156 141 147 153 149 5.76 3.8% Sample 2 984 848 932 931 923 48.70 5.2% Sample 2 984 848 932 931 923 48.70 5.2% Sample 3 3201 3180 3318 2761 3115 211.03 6.7% Sample 3 3201 3180 3318 2761 3115 211.03 6.7%
- Synonyms
- Plexin-B3; PLXNB3; KIAA1206; PLXN6
- Gene Name
- PLXNB3
- Gene ID
- 5365
- UniProt
- Q9ULL4
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: H45-Q1255
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expression detected in Purkinje and granular cells in cerebellum, and in brain neocortex but not in corpus callosum. Expressed in glioma cells and embryonic kidney cells (at protein level) . Expressed in brain, liver, pancreas and placenta, with weak expre
- Applications
- ELISA
- Field of Research
- Axonal Guidance Proteins, Growth and Development, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.6 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 984 3201 169 992 3321 Standard deviation 9.52 53.14 208.07 7.94 69.44 259.04 CV (%) 6.1% 5.4% 6.5% 4.7% 7.0% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 984 3201 169 992 3321 Standard deviation 9.52 53.14 208.07 7.94 69.44 259.04 CV (%) 6.1% 5.4% 6.5% 4.7% 7.0% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 156 984 3201 169 992 3321 Mean (pg/ml) 156 984 3201 169 992 3321 Standard deviation 9.52 53.14 208.07 7.94 69.44 259.04 Standard deviation 9.52 53.14 208.07 7.94 69.44 259.04 CV (%) 6.1% 5.4% 6.5% 4.7% 7.0% 7.8% CV (%) 6.1% 5.4% 6.5% 4.7% 7.0% 7.8%
- Sensitivity
- <15 pg/mL
- Function
- Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca2+/Mg2+-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human Plexin B3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Plexin B3
- Subcellular Location
- Cell membrane, Single-pass type I membrane protein
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.068 0.134 0.189 0.318 0.575 0.961 1.456 2.109 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.068 0.134 0.189 0.318 0.575 0.961 1.456 2.109 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.068 0.134 0.189 0.318 0.575 0.961 1.456 2.109 O.D. 0.068 0.134 0.189 0.318 0.575 0.961 1.456 2.109
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
