- Catalog number
- EK1840
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 9; 9 A4. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) . A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 54 55 49 54 3.24 6.0% Sample 2 270 320 291 297 294 17.81 6.0% Sample 3 884 818 866 930 874 40.10 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 54 55 49 54 3.24 6.0% Sample 1 58 54 55 49 54 3.24 6.0% Sample 2 270 320 291 297 294 17.81 6.0% Sample 2 270 320 291 297 294 17.81 6.0% Sample 3 884 818 866 930 874 40.10 4.5% Sample 3 884 818 866 930 874 40.10 4.5%
- Synonyms
- Junctional adhesion molecule C; JAM-C; JAM-2; Junctional adhesion molecule 3; JAM-3; Jam3
- Gene Name
- Jam3
- Gene ID
- 83964
- UniProt
- Q9D8B7
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E30-N241
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Endothelial cells.
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Platelets, Signal Transduction, Tags & Cell Markers, Vascular Inflammation, Vasculature
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 270 884 61 279 991 Standard deviation 2.90 14.04 39.78 2.81 17.86 74.33 CV (%) 5.0% 5.2% 4.5% 4.6% 6.4% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 270 884 61 279 991 Standard deviation 2.90 14.04 39.78 2.81 17.86 74.33 CV (%) 5.0% 5.2% 4.5% 4.6% 6.4% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 58 270 884 61 279 991 Mean (pg/ml) 58 270 884 61 279 991 Standard deviation 2.90 14.04 39.78 2.81 17.86 74.33 Standard deviation 2.90 14.04 39.78 2.81 17.86 74.33 CV (%) 5.0% 5.2% 4.5% 4.6% 6.4% 7.5% CV (%) 5.0% 5.2% 4.5% 4.6% 6.4% 7.5%
- Sensitivity
- <10 pg/mL
- Function
- Participates in cell-cell adhesion. It is a counter-receptor for ITGAM mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) . The soluble form is a mediator of angio
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse JAM-C/JAM3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Junction adhesion molecule 3
- Subcellular Location
- Cell membrane, Single-pass type I membrane protein, extracellular space, desmosome
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.096 0.131 0.207 0.363 0.646 1.115 2.016 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.096 0.131 0.207 0.363 0.646 1.115 2.016 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.096 0.131 0.207 0.363 0.646 1.115 2.016 O.D. 0.051 0.096 0.131 0.207 0.363 0.646 1.115 2.016
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
