- Catalog number
- EK1845
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- MANF is a 20kDa protein which belongs to the ARMET family. This gene is mapped to 3p21.2. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 156 148 167 169 160 8.51 5.3% Sample 2 733 654 771 713 717 42.29 5.8% Sample 3 2725 2678 2428 2740 2642 126.07 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 156 148 167 169 160 8.51 5.3% Sample 1 156 148 167 169 160 8.51 5.3% Sample 2 733 654 771 713 717 42.29 5.8% Sample 2 733 654 771 713 717 42.29 5.8% Sample 3 2725 2678 2428 2740 2642 126.07 4.7% Sample 3 2725 2678 2428 2740 2642 126.07 4.7%
- Synonyms
- Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; MANF; ARMET; ARP
- Gene Name
- MANF
- Gene ID
- 7873
- UniProt
- P55145
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L25-L182
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Amino Acids, Cancer, Cell Cycle, Cell Division, Neuroscience, Neurotransmitter
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.6 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2% 6.5% 4.6% 5.1% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2% 6.5% 4.6% 5.1% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2% 6.5% 4.6% 5.1% 7.4% 6.8% CV (%) 6.2% 6.5% 4.6% 5.1% 7.4% 6.8%
- Sensitivity
- <15 pg/mL
- Function
- Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents i
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human MANF
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Mesencephalic astrocyte derived neurotrophic factor
- Subcellular Location
- Endoplasmic reticulum lumen, Secreted, Sarcoplasmic reticulum lumen
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.089 0.129 0.242 0.459 0.854 1.64 2.229 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.089 0.129 0.242 0.459 0.854 1.64 2.229 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.089 0.129 0.242 0.459 0.854 1.64 2.229 O.D. 0.051 0.089 0.129 0.242 0.459 0.854 1.64 2.229
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
