- Catalog number
- EK1818
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Plexin-A1 is a protein that in humans is encoded by the PLXNA1 gene. This gene is mapped to 6; 6 D1. Plexin A1 has been shown to interact with AKT1.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 462 417 452 485 454 24.48 5.3% Sample 2 2424 2827 2492 2766 2627 172.30 6.5% Sample 3 8962 10130 10185 9716 9748 488.80 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 462 417 452 485 454 24.48 5.3% Sample 1 462 417 452 485 454 24.48 5.3% Sample 2 2424 2827 2492 2766 2627 172.30 6.5% Sample 2 2424 2827 2492 2766 2627 172.30 6.5% Sample 3 8962 10130 10185 9716 9748 488.80 5.0% Sample 3 8962 10130 10185 9716 9748 488.80 5.0%
- Synonyms
- Plexin-A1; Plex 1; Plexin-1; Plxna1; Kiaa4053
- Gene Name
- Plxna1
- Gene ID
- 18844
- UniProt
- P70206
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S28-P1242
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Ubiquitous.
- Applications
- ELISA
- Field of Research
- Axonal Guidance Proteins, Growth and Development, More Gpcr, Neurogenesis, Neurology Process, Neuroscience, Neurotransmission, Receptors / Channels
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 462 2424 8962 500 2463 9301 Standard deviation 21.71 123.62 412.25 27.00 155.17 697.58 CV (%) 4.7% 5.1% 4.6% 5.4% 6.3% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 462 2424 8962 500 2463 9301 Standard deviation 21.71 123.62 412.25 27.00 155.17 697.58 CV (%) 4.7% 5.1% 4.6% 5.4% 6.3% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 462 2424 8962 500 2463 9301 Mean (pg/ml) 462 2424 8962 500 2463 9301 Standard deviation 21.71 123.62 412.25 27.00 155.17 697.58 Standard deviation 21.71 123.62 412.25 27.00 155.17 697.58 CV (%) 4.7% 5.1% 4.6% 5.4% 6.3% 7.5% CV (%) 4.7% 5.1% 4.6% 5.4% 6.3% 7.5%
- Sensitivity
- <0.1 ng/mL
- Function
- Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex compos
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse Plexin A1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Plexin A1
- Subcellular Location
- Cell membrane, Single-pass type I membrane protein
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.067 0.154 0.192 0.272 0.492 0.732 1.391 2.245 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.067 0.154 0.192 0.272 0.492 0.732 1.391 2.245 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.067 0.154 0.192 0.272 0.492 0.732 1.391 2.245 O.D. 0.067 0.154 0.192 0.272 0.492 0.732 1.391 2.245
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
