- Catalog number
- EK1877
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. It is mapped to 10q11.21. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 148 147 153 148 149 2.34 1.5% Sample 2 628 656 611 669 641 22.79 3.5% Sample 3 2813 3203 3280 2837 3033 210.19 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 148 147 153 148 149 2.34 1.5% Sample 1 148 147 153 148 149 2.34 1.5% Sample 2 628 656 611 669 641 22.79 3.5% Sample 2 628 656 611 669 641 22.79 3.5% Sample 3 2813 3203 3280 2837 3033 210.19 6.9% Sample 3 2813 3203 3280 2837 3033 210.19 6.9%
- Synonyms
- Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret; RET; CDHF12; CDHR16; PTC; RET51
- Gene Name
- RET
- Gene ID
- 5979
- UniProt
- P07949
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L29-R635
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 93.8 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 148 628 2813 162 691 2933 Standard deviation 6.96 32.03 149.09 9.72 44.92 231.71 CV (%) 4.7% 5.1% 5.3% 6.0% 6.5% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 148 628 2813 162 691 2933 Standard deviation 6.96 32.03 149.09 9.72 44.92 231.71 CV (%) 4.7% 5.1% 5.3% 6.0% 6.5% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 148 628 2813 162 691 2933 Mean (pg/ml) 148 628 2813 162 691 2933 Standard deviation 6.96 32.03 149.09 9.72 44.92 231.71 Standard deviation 6.96 32.03 149.09 9.72 44.92 231.71 CV (%) 4.7% 5.1% 5.3% 6.0% 6.5% 7.9% CV (%) 4.7% 5.1% 5.3% 6.0% 6.5% 7.9%
- Sensitivity
- <15 pg/mL
- Function
- Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates P
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human RET
- Anticoagulant
- Heparin
- Gene Name Synonym
- Ret proto-oncogene
- Subcellular Location
- Endosome membrane, Single-pass type I membrane protein, Cell membrane
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.013 0.09 0.158 0.284 0.572 0.968 1.583 2.158 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.013 0.09 0.158 0.284 0.572 0.968 1.583 2.158 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.013 0.09 0.158 0.284 0.572 0.968 1.583 2.158 O.D. 0.013 0.09 0.158 0.284 0.572 0.968 1.583 2.158
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
