- Catalog number
- EK1881
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The C1QTNF9 gene is conserved in chimpanzee, Rhesus monkey, and zebrafish. This gene is mapped to 13q12.12. 42 organisms have orthologs with human gene C1QTNF9.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 59 68 62 63 3.35 5.3% Sample 2 280 327 277 285 292 20.26 6.9% Sample 3 1368 1367 1278 1292 1326 41.54 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 59 68 62 63 3.35 5.3% Sample 1 65 59 68 62 63 3.35 5.3% Sample 2 280 327 277 285 292 20.26 6.9% Sample 2 280 327 277 285 292 20.26 6.9% Sample 3 1368 1367 1278 1292 1326 41.54 3.1% Sample 3 1368 1367 1278 1292 1326 41.54 3.1%
- Synonyms
- Complement C1q and tumor necrosis factor-related protein 9A; Complement C1q and tumor necrosis factor-related protein 9; C1QTNF9; C1QTNF9A; UNQ6503; PRO21380
- Gene Name
- C1QTNF9
- Gene ID
- 338872
- UniProt
- P0C862
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q20-P333
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expressed predominantly in adipose tissue.
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Secreted Molecules, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 46.9 pg/mL - 3,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 280 1368 73 291 1425 Standard deviation 3.19 14.28 79.34 5.18 22.12 111.15 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 280 1368 73 291 1425 Standard deviation 3.19 14.28 79.34 5.18 22.12 111.15 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 65 280 1368 73 291 1425 Mean (pg/ml) 65 280 1368 73 291 1425 Standard deviation 3.19 14.28 79.34 5.18 22.12 111.15 Standard deviation 3.19 14.28 79.34 5.18 22.12 111.15 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8%
- Sensitivity
- <10 pg/mL
- Function
- Probable adipokine. Activates AMPK, AKT, and p44/42 MAPK signaling pathways.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CTRP9
- Anticoagulant
- Heparin
- Gene Name Synonym
- C1q and TNF related 9
- Subcellular Location
- Secreted
- Sample Data
- Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.024 0.117 0.193 0.372 0.646 1.049 1.787 2.421 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.024 0.117 0.193 0.372 0.646 1.049 1.787 2.421 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.024 0.117 0.193 0.372 0.646 1.049 1.787 2.421 O.D. 0.024 0.117 0.193 0.372 0.646 1.049 1.787 2.421
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
