- Catalog number
- EK1886
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 9q34. SERPINE2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa) .SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 413 438 416 463 432 20.08 4.6% Sample 2 2922 2913 2827 3068 2932 86.57 2.9% Sample 3 9164 8948 9570 8836 9129 280.31 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 413 438 416 463 432 20.08 4.6% Sample 1 413 438 416 463 432 20.08 4.6% Sample 2 2922 2913 2827 3068 2932 86.57 2.9% Sample 2 2922 2913 2827 3068 2932 86.57 2.9% Sample 3 9164 8948 9570 8836 9129 280.31 3.0% Sample 3 9164 8948 9570 8836 9129 280.31 3.0%
- Synonyms
- Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serpin E2; Serpine2; Pi7; Pn1
- Gene Name
- Serpine2
- Gene ID
- 29366
- UniProt
- P07092
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S20-P397
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Cell Adhesion Proteins, Cell Biology, Neurogenesis, Neurology Process, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate), urine.
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 413 2922 9164 595 3000 9451 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 CV (%) 4.2% 4.5% 7.1% 6.5% 5.5% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 413 2922 9164 595 3000 9451 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 CV (%) 4.2% 4.5% 7.1% 6.5% 5.5% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 413 2922 9164 595 3000 9451 Mean (pg/ml) 413 2922 9164 595 3000 9451 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 CV (%) 4.2% 4.5% 7.1% 6.5% 5.5% 7.8% CV (%) 4.2% 4.5% 7.1% 6.5% 5.5% 7.8%
- Sensitivity
- <0.1 ng/mL
- Function
- Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat SerpinE2
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Serpin family E member 2
- Subcellular Location
- Extracellular space
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.099 0.141 0.236 0.399 0.791 1.318 2.094 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.099 0.141 0.236 0.399 0.791 1.318 2.094 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.099 0.141 0.236 0.399 0.791 1.318 2.094 O.D. 0.052 0.099 0.141 0.236 0.399 0.791 1.318 2.094
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
