- Catalog number
- EK1835
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Regenerating islet-derived protein 3 alpha is a protein that in humans is encoded by the REG3A gene. It is mapped to 6 C3; 6 34.76 cM. This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 35 32 36 33 2.38 7.2% Sample 2 174 156 153 148 157 9.80 6.2% Sample 3 502 518 455 436 477 33.42 7.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 35 32 36 33 2.38 7.2% Sample 1 30 35 32 36 33 2.38 7.2% Sample 2 174 156 153 148 157 9.80 6.2% Sample 2 174 156 153 148 157 9.80 6.2% Sample 3 502 518 455 436 477 33.42 7.0% Sample 3 502 518 455 436 477 33.42 7.0%
- Synonyms
- Regenerating islet-derived protein 3-alpha; REG-3-alpha; Islet of Langerhans regenerating protein 3; Lithostathine 3; Pancreatitis-associated protein 2; Regenerating islet-derived protein III-alpha; Reg III-alpha; Reg3a; Pap2
- Gene Name
- Reg3a
- Gene ID
- 19694
- UniProt
- O09037
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: E27-Q175
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Small intestine and pancreas.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 174 502 32 209 528 Standard deviation 2.19 9.05 23.59 2.05 11.70 23.23 CV (%) 7.3% 5.2% 4.7% 6.4% 5.6% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 174 502 32 209 528 Standard deviation 2.19 9.05 23.59 2.05 11.70 23.23 CV (%) 7.3% 5.2% 4.7% 6.4% 5.6% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 174 502 32 209 528 Mean (pg/ml) 30 174 502 32 209 528 Standard deviation 2.19 9.05 23.59 2.05 11.70 23.23 Standard deviation 2.19 9.05 23.59 2.05 11.70 23.23 CV (%) 7.3% 5.2% 4.7% 6.4% 5.6% 4.4% CV (%) 7.3% 5.2% 4.7% 6.4% 5.6% 4.4%
- Sensitivity
- <10 pg/mL
- Function
- Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse REG3A
- Anticoagulant
- Heparin
- Gene Name Synonym
- Regenerating islet-derived 3 alpha
- Subcellular Location
- Secreted
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.063 0.107 0.148 0.22 0.415 0.721 1.182 1.941 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.063 0.107 0.148 0.22 0.415 0.721 1.182 1.941 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.063 0.107 0.148 0.22 0.415 0.721 1.182 1.941 O.D. 0.063 0.107 0.148 0.22 0.415 0.721 1.182 1.941
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
