- Catalog number
- EK1941
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- This gene is mapped to 1p36.23. The protein encoded by this gene is one of several isozymes of carbonic anhydrase. This protein is found only in salivary glands and saliva and protein may play a role in the reversible hydratation of carbon dioxide though its function in saliva is unknown.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 250 230 225 233 9.83 4.2% Sample 2 1372 1388 1260 1370 1347 50.99 3.7% Sample 3 5130 4907 4480 4360 4719 312.35 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 250 230 225 233 9.83 4.2% Sample 1 228 250 230 225 233 9.83 4.2% Sample 2 1372 1388 1260 1370 1347 50.99 3.7% Sample 2 1372 1388 1260 1370 1347 50.99 3.7% Sample 3 5130 4907 4480 4360 4719 312.35 6.6% Sample 3 5130 4907 4480 4360 4719 312.35 6.6%
- Synonyms
- Carbonic anhydrase 6; Carbonate dehydratase VI; Carbonic anhydrase VI; CA-VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase
- Gene Name
- CA6
- Gene ID
- 765
- UniProt
- P23280
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q18-N308
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Major constituent of saliva.
- Applications
- ELISA
- Field of Research
- Cell Biology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1372 5130 249 1437 5217 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1372 5130 249 1437 5217 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 228 1372 5130 249 1437 5217 Mean (pg/ml) 228 1372 5130 249 1437 5217 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1%
- Sensitivity
- <50 pg/mL
- Function
- Reversible hydration of carbon dioxide. Its role in saliva is unknown.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CA6
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Carbonic anhydrase 6
- Subcellular Location
- Secreted
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.152 0.242 0.417 0.743 1.221 1.826 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.152 0.242 0.417 0.743 1.221 1.826 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.152 0.242 0.417 0.743 1.221 1.826 O.D. 0.055 0.101 0.152 0.242 0.417 0.743 1.221 1.826
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
