- Catalog number
- EK1943
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined. It is mapped to 19q13.33.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 284 305 296 288 13.14 4.5% Sample 2 1292 1291 1147 1271 1250 60.19 4.8% Sample 3 4732 4272 4489 4168 4415 216.46 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 284 305 296 288 13.14 4.5% Sample 1 270 284 305 296 288 13.14 4.5% Sample 2 1292 1291 1147 1271 1250 60.19 4.8% Sample 2 1292 1291 1147 1271 1250 60.19 4.8% Sample 3 4732 4272 4489 4168 4415 216.46 4.9% Sample 3 4732 4272 4489 4168 4415 216.46 4.9%
- Synonyms
- C-type lectin domain family 11 member A; C-type lectin superfamily member 3; Lymphocyte secreted C-type lectin; Osteolectin; Stem cell growth factor; p47; CLEC11A; CLECSF3; LSLCL; SCGF
- Gene Name
- CLEC11A
- Gene ID
- 6320
- UniProt
- Q9Y240
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A22-F323
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expressed in skeletal tissues including bone marrow, chondrocytes, primary ossification center-associated cells, the perichondrium and periosteum. Lower levels of expression were detected in spleen, thymus, appendix and fetal liver.
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 1292 4732 284 1347 4986 Standard deviation 19.44 81.40 212.94 15.05 86.21 209.41 CV (%) 7.2% 6.3% 4.5% 5.3% 6.4% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 1292 4732 284 1347 4986 Standard deviation 19.44 81.40 212.94 15.05 86.21 209.41 CV (%) 7.2% 6.3% 4.5% 5.3% 6.4% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 270 1292 4732 284 1347 4986 Mean (pg/ml) 270 1292 4732 284 1347 4986 Standard deviation 19.44 81.40 212.94 15.05 86.21 209.41 Standard deviation 19.44 81.40 212.94 15.05 86.21 209.41 CV (%) 7.2% 6.3% 4.5% 5.3% 6.4% 4.2% CV (%) 7.2% 6.3% 4.5% 5.3% 6.4% 4.2%
- Sensitivity
- <50 pg/mL
- Function
- Promotes osteogenesis by stimulating the differentiation of mesenchymal progenitors into mature osteoblasts. Important for repair and maintenance of adult bone.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CLEC11A
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-type lectin domain containing 11A
- Subcellular Location
- Secreted, Cytoplasm
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.077 0.108 0.167 0.301 0.534 1.0 1.745 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.077 0.108 0.167 0.301 0.534 1.0 1.745 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.077 0.108 0.167 0.301 0.534 1.0 1.745 O.D. 0.042 0.077 0.108 0.167 0.301 0.534 1.0 1.745
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
