Mouse SMOC1 ELISA Kit PicoKine®

About this product

Mouse SMOC1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Smoc1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1936

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene is mapped to 12; 12 D1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 172 180 169 171 3.07 1.8% Sample 2 656 652 674 688 668 24.7 3.7% Sample 3 1364 1412 1531 1296 1401 81.24 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 172 180 169 171 3.07 1.8% Sample 1 162 172 180 169 171 3.07 1.8% Sample 2 656 652 674 688 668 24.7 3.7% Sample 2 656 652 674 688 668 24.7 3.7% Sample 3 1364 1412 1531 1296 1401 81.24 5.8% Sample 3 1364 1412 1531 1296 1401 81.24 5.8%

Synonyms

SPARC-related modular calcium-binding protein 1; SPARC-related gene protein; Secreted modular calcium-binding protein 1; SMOC-1; Smoc1; Srg

Gene Name

Smoc1

Gene ID

64075

UniProt

Q8BLY1

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H26-V463

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Widely expressed in many tissues with a strongest signal in ovary.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 656 1364 168 695 1436 Standard deviation 8.42 41.33 65.47 8.23 51.43 76.11 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 656 1364 168 695 1436 Standard deviation 8.42 41.33 65.47 8.23 51.43 76.11 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 656 1364 168 695 1436 Mean (pg/ml) 162 656 1364 168 695 1436 Standard deviation 8.42 41.33 65.47 8.23 51.43 76.11 Standard deviation 8.42 41.33 65.47 8.23 51.43 76.11 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3%

Sensitivity

<10 pg/mL

Function

Probable regulator of osteoblast differentiation. Plays essential roles in both eye and limb development.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SMOC1

Anticoagulant

Heparin

Gene Name Synonym

SPARC related modular calcium binding 1

Subcellular Location

Basement membrane

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.135 0.196 0.299 0.534 0.937 1.592 2.333 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.135 0.196 0.299 0.534 0.937 1.592 2.333 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.135 0.196 0.299 0.534 0.937 1.592 2.333 O.D. 0.07 0.135 0.196 0.299 0.534 0.937 1.592 2.333

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.