Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine®

About this product

Human CNDP1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CNDP1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1957

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Beta-Ala-His dipeptidase is an enzyme that in humans is encoded by the CNDP1 gene. It is mapped to 18q22.3. This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 5364 5541 5698 5319 5481 279.51 5.1% Sample 2 12789 12898 14236 13621 13386 736.23 5.5% Sample 3 25697 26546 26973 26459 26419 1241.68 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 5364 5541 5698 5319 5481 279.51 5.1% Sample 1 5364 5541 5698 5319 5481 279.51 5.1% Sample 2 12789 12898 14236 13621 13386 736.23 5.5% Sample 2 12789 12898 14236 13621 13386 736.23 5.5% Sample 3 25697 26546 26973 26459 26419 1241.68 4.7% Sample 3 25697 26546 26973 26459 26419 1241.68 4.7%

Synonyms

Beta-Ala-His dipeptidase; CNDP dipeptidase 1; Carnosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum carnosinase; CNDP1; CN1; CPGL2; UNQ1915/PRO4380

Gene Name

CNDP1

Gene ID

84735

UniProt

Q96KN2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-H507

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Found in serum and adult nervous central system. Absent in serum from patients with homocarnosinosis.

Applications

ELISA

Field of Research

Cancer, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction

Standard

200 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

6.25 ng/mL - 400 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 5364 12789 25697 5641 12786 25595 Standard deviation 193.1 537.14 1104.97 310.26 690.44 1377.74 CV (%) 3.6% 4.2% 4.3% 5.5% 5.4% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 5364 12789 25697 5641 12786 25595 Standard deviation 193.1 537.14 1104.97 310.26 690.44 1377.74 CV (%) 3.6% 4.2% 4.3% 5.5% 5.4% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 5364 12789 25697 5641 12786 25595 Mean (pg/ml) 5364 12789 25697 5641 12786 25595 Standard deviation 193.1 537.14 1104.97 310.26 690.44 1377.74 Standard deviation 193.1 537.14 1104.97 310.26 690.44 1377.74 CV (%) 3.6% 4.2% 4.3% 5.5% 5.4% 5.3% CV (%) 3.6% 4.2% 4.3% 5.5% 5.4% 5.3%

Sensitivity

<50 pg/mL

Function

Preferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CNDP1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Carnosine dipeptidase 1

Subcellular Location

Secreted

Sample Data

Concentration (ng/ml) 0 6.25 12.5 25 50 100 200 400 O.D. 0.089 0.162 0.219 0.355 0.575 0.97 1.64 2.074 Concentration (ng/ml) 0 6.25 12.5 25 50 100 200 400 O.D. 0.089 0.162 0.219 0.355 0.575 0.97 1.64 2.074 Concentration (ng/ml) 0 6.25 12.5 25 50 100 200 400 Concentration (ng/ml) 0 6.25 12.5 25 50 100 200 400 O.D. 0.089 0.162 0.219 0.355 0.575 0.97 1.64 2.074 O.D. 0.089 0.162 0.219 0.355 0.575 0.97 1.64 2.074

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.