- Catalog number
- EK1944
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 1p36.13. The protein encoded by this gene is a member of the phospholipase A2 family (PLA2) . PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5'UTRs have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 256 282 287 268 16.28 6.0% Sample 2 1511 1685 1589 1597 1595 61.63 3.8% Sample 3 3890 3984 4021 3994 3972 49.37 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 256 282 287 268 16.28 6.0% Sample 1 249 256 282 287 268 16.28 6.0% Sample 2 1511 1685 1589 1597 1595 61.63 3.8% Sample 2 1511 1685 1589 1597 1595 61.63 3.8% Sample 3 3890 3984 4021 3994 3972 49.37 1.2% Sample 3 3890 3984 4021 3994 3972 49.37 1.2%
- Synonyms
- Phospholipase A2, membrane associated; GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; PLA2G2A; PLA2B; PLA2L; RASF-A
- Gene Name
- PLA2G2A
- Gene ID
- 5320
- UniProt
- P14555
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N21-C144
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cardiovascular, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1511 3890 265 1585 4121 Standard deviation 13.20 93.68 171.16 18.82 76.08 259.62 CV (%) 5.3% 6.2% 4.4% 7.1% 4.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1511 3890 265 1585 4121 Standard deviation 13.20 93.68 171.16 18.82 76.08 259.62 CV (%) 5.3% 6.2% 4.4% 7.1% 4.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 249 1511 3890 265 1585 4121 Mean (pg/ml) 249 1511 3890 265 1585 4121 Standard deviation 13.20 93.68 171.16 18.82 76.08 259.62 Standard deviation 13.20 93.68 171.16 18.82 76.08 259.62 CV (%) 5.3% 6.2% 4.4% 7.1% 4.8% 6.3% CV (%) 5.3% 6.2% 4.4% 7.1% 4.8% 6.3%
- Sensitivity
- <50 pg/mL
- Function
- Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Binds to a site which is distinct from the classical ligand-binding site and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human PLA2G2A
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Phospholipase A2 group IIA
- Subcellular Location
- Secreted, Cell membrane, Peripheral membrane protein
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.074 0.127 0.226 0.48 0.822 1.297 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.074 0.127 0.226 0.48 0.822 1.297 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.074 0.127 0.226 0.48 0.822 1.297 2.024 O.D. 0.017 0.074 0.127 0.226 0.48 0.822 1.297 2.024
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
