- Catalog number
- EK1938
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family that encode carbonic anhydrase isozymes. It is mapped to 8q21.2. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1301 1297 1136 1156 1222 76.83 6.2% Sample 2 6028 5779 6552 5475 5958 394.67 6.6% Sample 3 24574 24410 24517 21425 23731 1332.95 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1301 1297 1136 1156 1222 76.83 6.2% Sample 1 1301 1297 1136 1156 1222 76.83 6.2% Sample 2 6028 5779 6552 5475 5958 394.67 6.6% Sample 2 6028 5779 6552 5475 5958 394.67 6.6% Sample 3 24574 24410 24517 21425 23731 1332.95 5.6% Sample 3 24574 24410 24517 21425 23731 1332.95 5.6%
- Synonyms
- Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III; CA-III; CA3
- Gene Name
- CA3
- Gene ID
- 761
- UniProt
- P07451
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A2-K260
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Muscle specific.
- Applications
- ELISA
- Field of Research
- Associated Proteins, Cardiovascular, Cell Biology, Cell Type Markers, Hypoxia
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1301 6028 24574 1319 6108 24732 Standard deviation 53.34 373.74 1105.83 61.99 390.91 1335.53 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1301 6028 24574 1319 6108 24732 Standard deviation 53.34 373.74 1105.83 61.99 390.91 1335.53 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1301 6028 24574 1319 6108 24732 Mean (pg/ml) 1301 6028 24574 1319 6108 24732 Standard deviation 53.34 373.74 1105.83 61.99 390.91 1335.53 Standard deviation 53.34 373.74 1105.83 61.99 390.91 1335.53 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4%
- Sensitivity
- <50 pg/mL
- Function
- Reversible hydration of carbon dioxide.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CA3
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Carbonic anhydrase 3
- Subcellular Location
- Cytoplasm
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384 O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
