Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine®

About this product

Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CA2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0681

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1174 1071 1108 1106 1114 37.23 3.3% Sample 2 5107 5193 4515 4791 4901 268.69 5.4% Sample 3 21947 21460 19663 20115 20796 937.16 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1174 1071 1108 1106 1114 37.23 3.3% Sample 1 1174 1071 1108 1106 1114 37.23 3.3% Sample 2 5107 5193 4515 4791 4901 268.69 5.4% Sample 2 5107 5193 4515 4791 4901 268.69 5.4% Sample 3 21947 21460 19663 20115 20796 937.16 4.5% Sample 3 21947 21460 19663 20115 20796 937.16 4.5%

Synonyms

Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II; CA2

Gene Name

CA2

Gene ID

760

UniProt

P00918

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-K260

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Alzheimer's Disease, Biochemicals, Cancer, Chemical Type, Diabetes, Heart Disease, Microbiology, Obesity

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1174 5107 21947 1365 6165 23984 Standard deviation 48.13 214.49 987.62 64.16 332.91 1367.09 CV (%) 4.1% 4.2% 4.5% 4.7% 5.4% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1174 5107 21947 1365 6165 23984 Standard deviation 48.13 214.49 987.62 64.16 332.91 1367.09 CV (%) 4.1% 4.2% 4.5% 4.7% 5.4% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1174 5107 21947 1365 6165 23984 Mean (pg/ml) 1174 5107 21947 1365 6165 23984 Standard deviation 48.13 214.49 987.62 64.16 332.91 1367.09 Standard deviation 48.13 214.49 987.62 64.16 332.91 1367.09 CV (%) 4.1% 4.2% 4.5% 4.7% 5.4% 5.7% CV (%) 4.1% 4.2% 4.5% 4.7% 5.4% 5.7%

Sensitivity

<50 pg/mL

Function

Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CA2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Carbonic anhydrase 2

Subcellular Location

Cell membrane, Cytoplasm

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.008 0.081 0.169 0.298 0.612 1.0 1.561 2.004 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.008 0.081 0.169 0.298 0.612 1.0 1.561 2.004 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.008 0.081 0.169 0.298 0.612 1.0 1.561 2.004 O.D. 0.008 0.081 0.169 0.298 0.612 1.0 1.561 2.004

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.