- Catalog number
- EK1962
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Coiled-coil domain-containing protein 80 is a protein that in humans is encoded by the CCDC80 gene. It is mapped to 3q13.2. Bioinformatics analysis suggests the CCDC80 protein is a peroxiredoxin.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 76 86 72 78 5.11 6.5% Sample2 359 370 404 430 390 28.08 7.2% Sample3 1459 1416 1530 1436 1460 43.04 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 76 86 72 78 5.11 6.5% Sample 1 79 76 86 72 78 5.11 6.5% Sample2 359 370 404 430 390 28.08 7.2% Sample2 359 370 404 430 390 28.08 7.2% Sample3 1459 1416 1530 1436 1460 43.04 2.9% Sample3 1459 1416 1530 1436 1460 43.04 2.9%
- Synonyms
- Coiled-coil domain-containing protein 80; Down-regulated by oncogenes protein 1; Up-regulated in BRS-3 deficient mouse homolog; CCDC80; DRO1; URB; HBE245
- Gene Name
- CCDC80
- Gene ID
- 151887
- UniProt
- Q76M96
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E22-Y950
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expressed in dermal papilla and dermal fibroblasts (at protein level) . Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts.
- Applications
- ELISA
- Field of Research
- Cancer, Developmental Biology, Mesenchymal Stem Cells, Metabolism, Neurology Process, Neuroscience, Organogenesis, Skeletal Development, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 46.9 pg/mL - 3,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 359 1459 86 438 1497 Standard deviation 3.32 24.05 80.25 5.85 25.84 91.32 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 359 1459 86 438 1497 Standard deviation 3.32 24.05 80.25 5.85 25.84 91.32 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 79 359 1459 86 438 1497 Mean (pg/ml) 79 359 1459 86 438 1497 Standard deviation 3.32 24.05 80.25 5.85 25.84 91.32 Standard deviation 3.32 24.05 80.25 5.85 25.84 91.32 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1%
- Sensitivity
- <10 pg/mL
- Function
- Promotes cell adhesion and matrix assembly.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human URB/CCDC80
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Coiled-coil domain containing 80
- Subcellular Location
- Extracellular matrix.
- Sample Data
- Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.021 0.086 0.145 0.276 0.535 1.014 1.701 2.472 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.021 0.086 0.145 0.276 0.535 1.014 1.701 2.472 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.021 0.086 0.145 0.276 0.535 1.014 1.701 2.472 O.D. 0.021 0.086 0.145 0.276 0.535 1.014 1.701 2.472
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
