- Catalog number
- EK1976
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fc receptor-like protein 2 is a protein that in humans is encoded by the FCRL2 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains one immunoreceptor-tyrosine activation motif and two immunoreceptor-tyrosine inhibitory motifs. This protein may be a prognostic marker for chronic lymphocytic leukemia. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 50 48 59 50 2.56 5.1% Sample 2 136 152 144 146 145 4.77 3.3% Sample 3 578 602 594 585 590 45.59 7.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 50 48 59 50 2.56 5.1% Sample 1 44 50 48 59 50 2.56 5.1% Sample 2 136 152 144 146 145 4.77 3.3% Sample 2 136 152 144 146 145 4.77 3.3% Sample 3 578 602 594 585 590 45.59 7.9% Sample 3 578 602 594 585 590 45.59 7.9%
- Synonyms
- Fc receptor-like protein 2; FcR-like protein 2; FcRL2; Fc receptor homolog 2; FcRH2; IFGP family protein 4; Immunoglobulin receptor translocation-associated protein 4; SH2 domain-containing phosphatase anchor protein 1; CD307b; FCRL2; FCRH2; IFGP4; IRTA4
- Gene Name
- FCRL2
- Gene ID
- 79368
- UniProt
- Q96LA5
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0, Immunogen sequence: L20-V401
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expressed in the secondary lymphoid organs, spleen and lymph node. Expression is limited to the mature B-cell lines. Highly expressed in CD19 and within the mantle zones of the tonsil tissue. Isoform 2 is expressed in the spleen, peripheral blood and bone
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 136 578 53 168 605 Standard deviation 3.17 8.57 37.17 2.39 7.06 32.07 CV (%) 7.2% 6.3% 6.4% 4.5% 4.2% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 136 578 53 168 605 Standard deviation 3.17 8.57 37.17 2.39 7.06 32.07 CV (%) 7.2% 6.3% 6.4% 4.5% 4.2% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 136 578 53 168 605 Mean (pg/ml) 44 136 578 53 168 605 Standard deviation 3.17 8.57 37.17 2.39 7.06 32.07 Standard deviation 3.17 8.57 37.17 2.39 7.06 32.07 CV (%) 7.2% 6.3% 6.4% 4.5% 4.2% 5.3% CV (%) 7.2% 6.3% 6.4% 4.5% 4.2% 5.3%
- Sensitivity
- <50 pg/mL
- Function
- May have an regulatory role in normal and neoplastic B cell development.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human FCRL2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fc receptor like 2
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.05 0.088 0.161 0.337 0.734 1.568 2.844 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.05 0.088 0.161 0.337 0.734 1.568 2.844 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.05 0.088 0.161 0.337 0.734 1.568 2.844 O.D. 0.016 0.05 0.088 0.161 0.337 0.734 1.568 2.844
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
