Human CD300C ELISA Kit PicoKine®

About this product

Human CD300C ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD300C in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1981

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

CMRF35-like molecule 6 (CLM-6) also known as CD300 antigen-like family member C (CD300c) is a protein that in humans is encoded by the CD300C gene. It is mapped to 17q25.1. The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 50 50 57 51 3.41 6.6% Sample 2 226 258 264 265 253 15.95 6.3% Sample 3 852 940 931 798 880 58.54 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 50 50 57 51 3.41 6.6% Sample 1 48 50 50 57 51 3.41 6.6% Sample 2 226 258 264 265 253 15.95 6.3% Sample 2 226 258 264 265 253 15.95 6.3% Sample 3 852 940 931 798 880 58.54 6.6% Sample 3 852 940 931 798 880 58.54 6.6%

Synonyms

CMRF35-like molecule 6; CLM-6; CD300 antigen-like family member C; CMRF35-A; CMRF-35; Immunoglobulin superfamily member 16; IgSF16; CD300c; CMRF35; CMRF35A; CMRF35A1; IGSF16

Gene Name

CD300C

Gene ID

10871

UniProt

Q08708

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G21-R183

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Present on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines.

Applications

ELISA

Field of Research

Adaptive Immunity, B Cells, Immunology, T Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 226 852 55 349 964 Standard deviation 2.98 15.14 38.34 3.25 24.78 41.45 CV (%) 6.2% 6.7% 4.5% 5.9% 7.1% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 226 852 55 349 964 Standard deviation 2.98 15.14 38.34 3.25 24.78 41.45 CV (%) 6.2% 6.7% 4.5% 5.9% 7.1% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 48 226 852 55 349 964 Mean (pg/ml) 48 226 852 55 349 964 Standard deviation 2.98 15.14 38.34 3.25 24.78 41.45 Standard deviation 2.98 15.14 38.34 3.25 24.78 41.45 CV (%) 6.2% 6.7% 4.5% 5.9% 7.1% 4.3% CV (%) 6.2% 6.7% 4.5% 5.9% 7.1% 4.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD300C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

CD300c molecule

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.076 0.123 0.221 0.403 0.754 1.313 2.535 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.076 0.123 0.221 0.403 0.754 1.313 2.535 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.076 0.123 0.221 0.403 0.754 1.313 2.535 O.D. 0.03 0.076 0.123 0.221 0.403 0.754 1.313 2.535

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.