- Catalog number
- EK1985
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties. This gene is mapped to 6p21.1.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 101 88 94 94 4.60 4.8% Sample 2 472 395 417 458 435 30.90 7.1% Sample 3 1928 1838 1770 2074 1902 113.77 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 101 88 94 94 4.60 4.8% Sample 1 94 101 88 94 94 4.60 4.8% Sample 2 472 395 417 458 435 30.90 7.1% Sample 2 472 395 417 458 435 30.90 7.1% Sample 3 1928 1838 1770 2074 1902 113.77 5.9% Sample 3 1928 1838 1770 2074 1902 113.77 5.9%
- Synonyms
- Trem-like transcript 2 protein; TLT-2; Triggering receptor expressed on myeloid cells-like protein 2; TREML2; C6orf76, TLT2; UNQ6268/PRO20473
- Gene Name
- TREML2
- Gene ID
- 79865
- UniProt
- Q5T2D2
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: G19-S268
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Detected in cultured B-cells, T-cell leukemia and monocyte leukemia. Expressed constitutively on CD8 T-cells and induced on CD4 T-cells after activation.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Immunology, Innate Immunity, Macrophage/Inflammation, Non-CD, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 472 1928 111 507 1976 Standard deviation 6.30 24.54 109.90 6.99 27.38 81.02 CV (%) 6.7% 5.2% 5.7% 6.3% 5.4% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 472 1928 111 507 1976 Standard deviation 6.30 24.54 109.90 6.99 27.38 81.02 CV (%) 6.7% 5.2% 5.7% 6.3% 5.4% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 94 472 1928 111 507 1976 Mean (pg/ml) 94 472 1928 111 507 1976 Standard deviation 6.30 24.54 109.90 6.99 27.38 81.02 Standard deviation 6.30 24.54 109.90 6.99 27.38 81.02 CV (%) 6.7% 5.2% 5.7% 6.3% 5.4% 4.1% CV (%) 6.7% 5.2% 5.7% 6.3% 5.4% 4.1%
- Sensitivity
- <50 pg/mL
- Function
- Cell surface receptor that may play a role in the innate and adaptive immune response. Acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human TREmL2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Triggering receptor expressed on myeloid cells like 2
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.082 0.126 0.207 0.344 0.588 1.047 1.942 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.082 0.126 0.207 0.344 0.588 1.047 1.942 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.082 0.126 0.207 0.344 0.588 1.047 1.942 O.D. 0.036 0.082 0.126 0.207 0.344 0.588 1.047 1.942
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
