- Catalog number
- EK1983
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cysteine-rich with EGF-like domain protein 2 is a protein that in humans is encoded by the CRELD2 gene. It is mapped to 22q13.33. CRELD2 was identified by searching a sequence database using CRELD1 as the query. Like CRELD1, with which it shares 38% amino acid identity, the deduced 321-amino acid CRELD2 protein contains EGF-like repeats and a highly conserved domain rich in glutamic acid and tryptophan (WE domain) . The major distinction between CRELD1 and CRELD2 is that CRELD1 has 2 type III transmembrane domains in its C terminus, while CRELD2 has none. CRELD2 shares 67% and 69% amino acid identity with its Chinese hamster and mouse homologs, respectively.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 153 154 147 158 153 3.93 2.5% Sample 2 696 700 750 711 714 21.35 2.9% Sample 3 3699 3606 3621 3798 3681 76.22 2.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 153 154 147 158 153 3.93 2.5% Sample 1 153 154 147 158 153 3.93 2.5% Sample 2 696 700 750 711 714 21.35 2.9% Sample 2 696 700 750 711 714 21.35 2.9% Sample 3 3699 3606 3621 3798 3681 76.22 2.0% Sample 3 3699 3606 3621 3798 3681 76.22 2.0%
- Synonyms
- Cysteine-rich with EGF-like domain protein 2; CRELD2; UNQ185/PRO211
- Gene Name
- CRELD2
- Gene ID
- 79174
- UniProt
- Q6UXH1
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A25-L353
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Ubiquitously expressed.
- Applications
- ELISA
- Field of Research
- Cell Biology, Ligand-Gated Ion Channels, Neuroscience, Neurotransmission, Receptors / Channels
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 153 696 3699 209 874 4132 Standard deviation 6.43 42.46 240.44 11.29 37.58 231.39 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 153 696 3699 209 874 4132 Standard deviation 6.43 42.46 240.44 11.29 37.58 231.39 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 153 696 3699 209 874 4132 Mean (pg/ml) 153 696 3699 209 874 4132 Standard deviation 6.43 42.46 240.44 11.29 37.58 231.39 Standard deviation 6.43 42.46 240.44 11.29 37.58 231.39 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6%
- Sensitivity
- <50 pg/mL
- Function
- May regulate transport of alpha4-beta2 neuronal acetylcholine receptor.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CRELD2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cysteine rich with EGF like domains 2
- Subcellular Location
- Secreted. Endoplasmic reticulum.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.169 0.28 0.471 0.805 1.403 2.042 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.169 0.28 0.471 0.805 1.403 2.042 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.169 0.28 0.471 0.805 1.403 2.042 O.D. 0.045 0.111 0.169 0.28 0.471 0.805 1.403 2.042
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
