- Catalog number
- EK1980
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD300A (Cluster of Differentiation 300A) is a human gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 362 424 405 365 389 26.39 6.7% Sample 2 1768 1661 1621 1762 1703 63.62 3.7% Sample 3 9786 8596 9892 9589 9465 513.78 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 362 424 405 365 389 26.39 6.7% Sample 1 362 424 405 365 389 26.39 6.7% Sample 2 1768 1661 1621 1762 1703 63.62 3.7% Sample 2 1768 1661 1621 1762 1703 63.62 3.7% Sample 3 9786 8596 9892 9589 9465 513.78 5.4% Sample 3 9786 8596 9892 9589 9465 513.78 5.4%
- Synonyms
- CMRF35-like molecule 8; CLM-8; CD300 antigen-like family member A; CMRF-35-H9; CMRF35-H9; CMRF35-H; IRC1/IRC2; Immunoglobulin superfamily member 12; IgSF12; Inhibitory receptor protein 60; IRp60; NK inhibitory receptor; CD300a; CD300A; CMRF35H; IGSF12; HS
- Gene Name
- CD300A
- Gene ID
- 11314
- UniProt
- Q9UGN4
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L18-P180
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Immunology, Innate Immunity, Macrophage/Inflammation, NK Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 362 1768 9786 405 1772 9892 Standard deviation 218.82 111.38 469.73 19.85 131.13 524.28 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 362 1768 9786 405 1772 9892 Standard deviation 218.82 111.38 469.73 19.85 131.13 524.28 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 362 1768 9786 405 1772 9892 Mean (pg/ml) 362 1768 9786 405 1772 9892 Standard deviation 218.82 111.38 469.73 19.85 131.13 524.28 Standard deviation 218.82 111.38 469.73 19.85 131.13 524.28 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3%
- Sensitivity
- <10 pg/mL
- Function
- Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but no
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CD300A
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD300a molecule
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.117 0.18 0.306 0.517 0.883 1.64 2.758 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.117 0.18 0.306 0.517 0.883 1.64 2.758 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.117 0.18 0.306 0.517 0.883 1.64 2.758 O.D. 0.046 0.117 0.18 0.306 0.517 0.883 1.64 2.758
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
