- Catalog number
- EK1991
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interferon-alpha/beta receptor alpha chain is a protein that in humans is encoded by the IFNAR1 gene. It is mapped to 16 C3.3; 16 52.98 cM. The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 123 112 114 105 113 6.42 5.6% Sample 2 489 411 498 489 471 35.26 7.4% Sample 3 1822 1958 1775 1754 1827 79.40 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 123 112 114 105 113 6.42 5.6% Sample 1 123 112 114 105 113 6.42 5.6% Sample 2 489 411 498 489 471 35.26 7.4% Sample 2 489 411 498 489 471 35.26 7.4% Sample 3 1822 1958 1775 1754 1827 79.40 4.3% Sample 3 1822 1958 1775 1754 1827 79.40 4.3%
- Gene Name
- IFNAR1
- Gene ID
- 15975
- UniProt
- P33896
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E27-T429
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interferons, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin or EDTA)
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 489 1822 124 492 1897 Standard deviation 5.04 22.98 72.88 7.07 26.08 121.41 CV (%) 4.1% 4.7% 4.0% 5.7% 5.3% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 489 1822 124 492 1897 Standard deviation 5.04 22.98 72.88 7.07 26.08 121.41 CV (%) 4.1% 4.7% 4.0% 5.7% 5.3% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 123 489 1822 124 492 1897 Mean (pg/ml) 123 489 1822 124 492 1897 Standard deviation 5.04 22.98 72.88 7.07 26.08 121.41 Standard deviation 5.04 22.98 72.88 7.07 26.08 121.41 CV (%) 4.1% 4.7% 4.0% 5.7% 5.3% 6.4% CV (%) 4.1% 4.7% 4.0% 5.7% 5.3% 6.4%
- Sensitivity
- <10 pg/mL
- Function
- Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1. Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves. Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway. Contributes to modulate the innate immune response to bacterial lipopolysaccharide.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse IFNAR1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interferon (alpha and beta) receptor 1
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein. Late endosome. Lysosome.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.047 0.112 0.151 0.276 0.509 0.905 1.549 2.294 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.047 0.112 0.151 0.276 0.509 0.905 1.549 2.294 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.047 0.112 0.151 0.276 0.509 0.905 1.549 2.294 O.D. 0.047 0.112 0.151 0.276 0.509 0.905 1.549 2.294
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
